抗Vimentin [J144]抗体 | Anti-Vimentin [J144] antibody

• 特長
• 価格
• DATA IMAGES
• 製品情報
• APPLICATION
• PROPERTIES
• TARGET

特長

• 高品質の抗体です。
• 幅広い研究分野に関連する抗体を取り揃えています。
• 使用されたアプリケーションや動物種などの情報が充実しています。
• 多数の論文で使用実績がある信頼性の高い抗体です。

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価格

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DATA IMAGES

	ICC/IF analysis of HEK293 cells using GTX80814 Vimentin antibody [J144]. Cells were probed without (right) or with(left) an antibody.Green : Primary antibodyBlue : NucleiRed : ActinFixation : formaldehydeDilution : 1:200 overnight at 4ºC
	ICC/IF analysis of HeLa cells untreated or treated with 1uM Actinomycin D for 19 hours using GTX80814 Vimentin antibody [J144]. Fixation : 4% paraformaldehydePermeabilization : 0.1% Triton X-100 for 10 minutes
	IHC-P analysis of human melanoma tissue using GTX80814 Vimentin antibody [J144]. Left : Primary antibodyRight : Negative control without primary antibodyAntigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutesDilution : 1:1000
	IHC-P analysis of human tonsil tissue using GTX80814 Vimentin antibody [J144]. Left : Primary antibodyRight : Negative control without primary antibodyAntigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutesDilution : 1:1000
	ICC/IF analysis of HeLa cells using GTX80814 Vimentin antibody [J144]. Cells were probed without (right) or with(left) an antibody.Green : Primary antibodyBlue : NucleiRed : ActinFixation : formaldehydeDilution : 1:200 overnight at 4ºC
	ICC/IF analysis of C6 cells using GTX80814 Vimentin antibody [J144]. Cells were probed without (right) or with(left) an antibody.Green : Primary antibodyBlue : NucleiRed : ActinFixation : formaldehydeDilution : 1:200 overnight at 4ºC
	WB analysis of 20 ug of indicated whole cell lysates using GTX80814 Vimentin antibody [J144]. Dilution : 1:500

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製品情報

Host	Mouse
Clonality	Monoclonal
Clone Name	J144
Isotype	IgM
Application	WB, ICC/IF, IHC-P, IHC
Reactivity	Human, Mouse, Rat, Dog

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APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Dilution
WB	1:1,000
ICC/IF	1:20-1:200
IHC-P	1:20
IHC	Assay dependent

Not tested in other applications.

Calculated MW	54 kDa. ( Note )

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PROPERTIES

Form	Liquid
Buffer	Ascites, 0.05% sodium azide
Storage	Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Antigen Species	Human
Immunogen	Human rhabdosarcoma cell line JR1.
Purification	Unpurified
Conjugation	Unconjugated
Note	For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

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TARGET

Synonyms	vimentin
Cellular Localization	Cytoplasm,Cytoplasm, cytoskeleton
Background	This gene encodes a type III intermediate filament protein. Intermediate filaments, along with microtubules and actin microfilaments, make up the cytoskeleton. The encoded protein is responsible for maintaining cell shape and integrity of the cytoplasm, and stabilizing cytoskeletal interactions. This protein is involved in neuritogenesis and cholesterol transport and functions as an organizer of a number of other critical proteins involved in cell attachment, migration, and signaling. Bacterial and viral pathogens have been shown to attach to this protein on the host cell surface. Mutations in this gene are associated with congenital cataracts in human patients. [provided by RefSeq, Aug 2017]
Database	・ Gene ID: 7431 VIM ・ UniProt: P08670 VIM

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