抗Histone H2A.XS139ph (phospho Ser139) [3F2]抗体 | Anti-Histone H2A.XS139ph (phospho Ser139) [3F2] antibody

• 特長
• 価格
• DATA IMAGES
• 製品情報
• APPLICATION
• PROPERTIES
• TARGET
• REFERENCEE(抜粋)

特長

• 高品質の抗体です。
• 幅広い研究分野に関連する抗体を取り揃えています。
• 使用されたアプリケーションや動物種などの情報が充実しています。
• 多数の論文で使用実績がある信頼性の高い抗体です。

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価格

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DATA IMAGES

	ICC/IF analysis of gamma irradiated HeLa cells using GTX80694 Histone H2A.XS139ph (phospho Ser139) antibody [3F2].
	ICC/IF analysis of cells using GTX80694 Histone H2A.XS139ph (phospho Ser139) antibody [3F2].
	WB analysis of Jurkat cell untreated (Lane 1) and Jurkat cell stimulated with staurosporine (Lane 2) using GTX80694 Histone H2A.XS139ph (phospho Ser139) antibody [3F2]. Dilution : 1 μg/ml
	ICC/IF analysis of A549 cells treated with vehicle (control; 0.1% DMSO in media) or with 50 μM etoposide for 1 hour using GTX80694 Histone H2A.XS139ph (phospho Ser139) antibody [3F2]. Red : Primary antibodyBlue : Nuclei
	IHC-P analysis of postnatal mouse (PN19) lung sections using GTX80694 Histone H2A.XS139ph (phospho Ser139) antibody [3F2]. Increased staining intensity was observed in a genetic mouse model with DNA damage in airway cells.Antigen retrieval : heat induced epitope retrieval (HIER) method with sodium citrate buffer (pH 6.0)Fixation : 4% ParaformaldehydeDilution : 1:400

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製品情報

Host	Mouse
Clonality	Monoclonal
Clone Name	3F2
Isotype	IgG1
Application	WB, ICC/IF, IHC-P, FACS, ELISA
Reactivity	Human, Mouse, Bovine

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APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Dilution
WB	1 μg/ml
ICC/IF	2-4 μg/ml
IHC-P	1:400
FACS	1μg per 10⁶ cells
ELISA	Assay dependent

Not tested in other applications.

Calculated MW	15 kDa. ( Note )
Specificity/Sensitivity	The immunogen of this antibody is a synthetic peptide sequence surrounding phosphorylated Ser140. Some references call Ser140, by the name Ser139.

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PROPERTIES

Form	Liquid
Buffer	PBS, 1mg/ml BSA, 0.05% sodium azide
Storage	Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration	1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species	Human
Immunogen	Synthetic peptide sequence surrounding phosphorylated Ser140
Purification	Protein G purified
Conjugation	Unconjugated
Note	For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

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TARGET

Synonyms	H2A histone family member X , H2A.X , H2A/X , H2AX
Cellular Localization	Nucleus
Background	Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene encodes a replication-independent histone that is a member of the histone H2A family, and generates two transcripts through the use of the conserved stem-loop termination motif, and the polyA addition motif. [provided by RefSeq, Oct 2015]
Database	・ Gene ID: 3014 H2AFX ・ UniProt: P16104 H2AFX

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REFERENCEE(抜粋)

Application Reference	Application/Reactivity
Wang YY et al. Cancer Chemother Pharmacol 2017; 79 (6) : 1129-1140 CYT-Rx20 inhibits ovarian cancer cells in vitro and in vivo through oxidative stress-induced DNA damage and cell apoptosis.	Application : WB Reactivity : Human
Wang YY et al. Head Neck 2017; (Epub) Synthetic β-nitrostyrene derivative CYT-Rx20 as inhibitor of oral cancer cell proliferation and tumor growth through glutathione suppression and reactive oxygen species induction.	Application : WB Reactivity : Human
Shang HS et al. Oncol Rep 2016; 36 (4) : 2207-15 Curcumin causes DNA damage and affects associated protein expression in HeLa human cervical cancer cells.
Smith S et al. PLoS One 2014; 9 (1) : e87203 Histone deacetylase inhibitors selectively target homology dependent DNA repair defective cells and elevate non-homologous endjoining activity.	Application : WB Reactivity : Human
Lee JH et al. PLoS One 2014; 9 (6) : e97969 Direct Activation of ATM by Resveratrol under Oxidizing Conditions.	Application : ICC/IF Reactivity : Human

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