抗beta Catenin [9F2]抗体 | Anti-beta Catenin [9F2] antibody

• 特長
• 価格
• DATA IMAGES
• 製品情報
• APPLICATION
• PROPERTIES
• TARGET

特長

• 高品質の抗体です。
• 幅広い研究分野に関連する抗体を取り揃えています。
• 使用されたアプリケーションや動物種などの情報が充実しています。
• 多数の論文で使用実績がある信頼性の高い抗体です。

目次に戻る

価格

目次に戻る

DATA IMAGES

	WB analysis of whole cell extracts (30 μg lysate) of HeLa (Lane 1), U-87 MG (Lane 2), COLO 205 (Lane 3), HCT 116 (lane 4), HT-29 (lane 5), MCF7 (lane 6), MDA-MB-231 (lane 7) and A549 (lane 8) using GTX80686 beta Catenin antibody [9F2]. Dilution : 1-3 μg/ml
	ICC/IF analysis of HeLa cells using GTX80686 beta Catenin antibody [9F2]. Cells were probed without (right) or with(left) an antibody.Green : Primary antibodyBlue : NucleiRed : ActinFixation : formaldehydeDilution : 1:20 overnight at 4ºC
	ICC/IF analysis of U251 cells using GTX80686 beta Catenin antibody [9F2]. Cells were probed without (right) or with(left) an antibody.Green : Primary antibodyBlue : NucleiRed : ActinFixation : formaldehydeDilution : 1:20 overnight at 4ºC

目次に戻る

製品情報

Host	Mouse
Clonality	Monoclonal
Clone Name	9F2
Isotype	IgG1
Application	WB, ICC/IF, IP
Reactivity	Human, Mouse

目次に戻る

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Dilution
WB	1-3 μg/ml
ICC/IF	10 μg/ml
IP	1 μg/ml

Not tested in other applications.

Calculated MW	85 kDa. ( Note )

目次に戻る

PROPERTIES

Form	Liquid
Buffer	PBS, 1mg/ml BSA, 0.05% sodium azide
Storage	Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration	1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species	Human
Immunogen	120 kDa MBP/beta catenin fusion protein purified on amylose column.
Purification	Protein G purified
Conjugation	Unconjugated
Note	For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

目次に戻る

TARGET

Synonyms	catenin beta 1 , CTNNB , EVR7 , MRD19 , armadillo
Cellular Localization	Cytoplasm,Nucleus,Cytoplasm, cytoskeleton,Cell junction,Cell membrane,Cell junction, synapse
Background	The protein encoded by this gene is part of a complex of proteins that constitute adherens junctions (AJs). AJs are necessary for the creation and maintenance of epithelial cell layers by regulating cell growth and adhesion between cells. The encoded protein also anchors the actin cytoskeleton and may be responsible for transmitting the contact inhibition signal that causes cells to stop dividing once the epithelial sheet is complete. Finally, this protein binds to the product of the APC gene, which is mutated in adenomatous polyposis of the colon. Mutations in this gene are a cause of colorectal cancer (CRC), pilomatrixoma (PTR), medulloblastoma (MDB), and ovarian cancer. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2016]
Database	・ Gene ID: 1499 CTNNB1 ・ UniProt: P35222 CTNNB1

目次に戻る