抗PSD95 [7E3-1B8]抗体 | Anti-PSD95 [7E3-1B8] antibody

• 特長
• 価格
• DATA IMAGES
• 製品情報
• APPLICATION
• PROPERTIES
• TARGET
• REFERENCEE(抜粋)

特長

• 高品質の抗体です。
• 幅広い研究分野に関連する抗体を取り揃えています。
• 使用されたアプリケーションや動物種などの情報が充実しています。
• 多数の論文で使用実績がある信頼性の高い抗体です。

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価格

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DATA IMAGES

	FACS analysis of U87-MG cells using GTX80682 PSD95 antibody [7E3-1B8] compared to an isotype control (blue).Dilution : 2 ug/test for 60 min at room temperatureFixation : 2% paraformaldehyde
	ICC/IF analysis of rat hippocampal cells using GTX80682 PSD95 antibody [7E3-1B8].
	FACS analysis of Neuro-2a cells using GTX80682 PSD95 antibody [7E3-1B8] compared to an isotype control (blue).Dilution : 2 ug/test for 60 min at room temperatureFixation : 2% paraformaldehyde
	ICC/IF analysis of C6 cells using GTX80682 PSD95 antibody [7E3-1B8]. Cells were probed without (right) or with(left) an antibody.Green : Primary antibodyBlue : NucleiRed : ActinFixation : formaldehydeDilution : 1:20 overnight at 4ºC
	WB analysis of 25 ug of mouse brain (lane 1) and rat brain (lane 2) cell lysates using GTX80682 PSD95 antibody [7E3-1B8]. Dilution : 1:200
	FACS analysis of SH-SY5Y cells using GTX80682 PSD95 antibody [7E3-1B8] compared to an isotype control (blue).Dilution : 2 ug/test for 60 min at room temperatureFixation : 2% paraformaldehyde
	ICC/IF analysis of HeLa cells using GTX80682 PSD95 antibody [7E3-1B8]. Cells were probed without (right) or with(left) an antibody.Green : Primary antibodyBlue : NucleiRed : ActinFixation : formaldehydeDilution : 1:20 overnight at 4ºC
	ICC/IF analysis of cells using GTX80682 PSD95 antibody [7E3-1B8].

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製品情報

Host	Mouse
Clonality	Monoclonal
Clone Name	7E3-1B8
Isotype	IgG1
Application	WB, ICC/IF, IHC-Fr, FACS, IP, IHC, Neutralizing/Blocking
Reactivity	Human, Mouse, Rat, Zebrafish, Rabbit, Bovine, Chicken, Marsupials, Xenopus, Primate

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APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Dilution
WB	1:2,000
ICC/IF	1:500
IHC-Fr	Assay dependent
FACS	2 μg/test
IP	Assay dependent
IHC	Assay dependent
Neutralizing/Blocking	Assay dependent

Not tested in other applications.

Calculated MW	80 kDa. ( Note )

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PROPERTIES

Form	Liquid
Buffer	PBS, 0.05% sodium azide
Storage	Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration	1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species	Rat
Immunogen	Purified recombinant rat PSD-95.
Purification	Protein A purified
Conjugation	Unconjugated
Note	For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

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TARGET

Synonyms	discs large MAGUK scaffold protein 4 , Dlgh4 , PSD95 , Sap90
Cellular Localization	Cell membrane,Cell junction, synapse,Cytoplasm
Background	a molecular scaffolding protein that binds and clusters N-methyl-D-aspartate receptors at neuronal synapses; may be involved in guanine nucleotide-mediated signal transduction pathway [RGD, Feb 2006]
Database	・ Gene ID: 29495 Dlg4 ・ UniProt: P31016 Dlg4

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REFERENCEE(抜粋)

Application Reference	Application/Reactivity
Mans RA et al. Front Mol Neurosci 2019; 12 : 91 Cholinergic Stimulation of the Adult Zebrafish Brain Induces Phosphorylation of Glycogen Synthase Kinase-3 帣 and Extracellular Signal-Regulated Kinase in the Telencephalon.
Jain T et al. Mol Pharmacol 2013; 84 (2) : 296-302 Diacylglycerol lipaseα (DAGLα) and DAGLβ cooperatively regulate the production of 2-arachidonoyl glycerol in autaptic hippocampal neurons.	Application : IHC Reactivity : Mouse
Hu SS et al. J Comp Neurol 2010; 518 (18) : 3848-66 Architecture of cannabinoid signaling in mouse retina.	Application : IHC Reactivity : Mouse
Honjo Y et al. Genes Cells. 2000; 5 (4):309-18 Blockade of cadherin-6B activity perturbs the distribution of PSD-95 family proteins in retinal neurones.	Application : ICC/IF Reactivity : Chicken

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