抗HLA-G [87G] (PE)抗体 | Anti-HLA-G [87G] (PE) antibody

• 特長
• 価格
• DATA IMAGES
• 製品情報
• APPLICATION
• PROPERTIES
• TARGET

特長

• 高品質の抗体です。
• 幅広い研究分野に関連する抗体を取り揃えています。
• 使用されたアプリケーションや動物種などの情報が充実しています。
• 多数の論文で使用実績がある信頼性の高い抗体です。

目次に戻る

価格

目次に戻る

DATA IMAGES

Analysis of cytolytical activity of human polyclonal NK cells on target melanoma cells. Blocking of HLA-G1 on transfectants with anti-human HLA-G (87G) restored specific lysis. Target cells: M8 cell line transfected with empty vector (column 1) and with HLA-G1 cDNA (columns 2-6).Blocking antibodies: Column 1-2: noneColumn 3: Isotype mouse IgG2a controlColumn 4: anti-human HLA-G (87G) purifiedColumn 5: anti-human HLA-G (87G) F(ab')2 fragmentColumn 6: anti-human HLA-G (MEM-G/9)

目次に戻る

製品情報

Host	Mouse
Clonality	Monoclonal
Clone Name	87G
Isotype	IgG2a
Application	WB, FACS
Reactivity	Human

目次に戻る

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Dilution
WB	Assay dependent
FACS	Assay dependent

Not tested in other applications.

Calculated MW	38 kDa. ( Note )
Specificity/Sensitivity	This antibody recognizes both membrane-bound and soluble forms of HLA-G (HLA-G1 and HLA-G5). The antibody 87G blocks interaction of HLA-G with inhibitory receptors. This product does not react with Mouse,Rat.

目次に戻る

PROPERTIES

Form	Liquid
Buffer	PBS, 15mM sodium azide
Storage	Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC. DO NOT FREEZE. Protect from light.
Concentration	0.1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species	Human
Immunogen	HLA-B27 transgenic mice were imunized with H-2 identical murine cells transfected with and expressing genes encoding HLA-G and human beta2-microglobulin.
Purification	Purified by size-exclusion chromatography
Conjugation	Phycoerythrin (PE)
Note	For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

目次に戻る

TARGET

Synonyms	HLAG,MHCG,major histocompatibility complex, class I, G,HLA-G
Cellular Localization	Cell membrane,Secreted
Background	HLA-G belongs to the HLA class I heavy chain paralogues. This class I molecule is a heterodimer consisting of a heavy chain and a light chain (beta-2 microglobulin). The heavy chain is anchored in the membrane. HLA-G is expressed on fetal derived placental cells. The heavy chain is approximately 45 kDa and its gene contains 8 exons. Exon one encodes the leader peptide, exons 2 and 3 encode the alpha1 and alpha2 domain, which both bind the peptide, exon 4 encodes the alpha3 domain, exon 5 encodes the transmembrane region, and exon 6 encodes the cytoplasmic tail. [provided by RefSeq, Jul 2008]
Database	・ Gene ID: 3135 HLA-G ・ UniProt: P17693 HLA-G

目次に戻る