抗EGFR (phospho Tyr1173) [EM-13]抗体 | Anti-EGFR (phospho Tyr1173) [EM-13] antibody

• 特長
• 価格
• DATA IMAGES
• 製品情報
• APPLICATION
• PROPERTIES
• TARGET

特長

• 高品質の抗体です。
• 幅広い研究分野に関連する抗体を取り揃えています。
• 使用されたアプリケーションや動物種などの情報が充実しています。
• 多数の論文で使用実績がある信頼性の高い抗体です。

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価格

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DATA IMAGES

	WB analysis of EGF-treated A431 (A), CALU-3 (B), MCF-7 (C), Jurkat (D) and Ramos (E) cell lines using GTX80161 EGFR (phospho Tyr1173) antibody [EM-13].
	IP analysis of EGF-treated A431 cells using phosphospecific EGFR antibodies GTX80162(A), GTX80161(B) , a commercial anti-EGFR polyclonal antibody(C), and anti-EGFR monoclonal mAb108 (D). The precipitates were immunoblotted with a commercial anti-EGFR polyclonal antibody.
	IP analysis of EGF-treated A431 cells using phosphospecific EGFR antibodies ,GTX80162 (A), GTX80161 (B) , a commercial anti-EGFR polyclonal antibody(C), and anti-EGFR monoclonal mAb108 (D). The precipitates were immunoblotted with GTX80162 EGFR (phospho Tyr992) antibody [EM-12] or GTX80161 EGFR (phospho Tyr1173) antibody [EM-13] antibody.

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製品情報

Host	Mouse
Clonality	Monoclonal
Clone Name	EM-13
Isotype	IgG1
Application	WB, FACS, IP
Reactivity	Human

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APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Dilution
WB	Assay dependent
FACS	Assay dependent
IP	Assay dependent

Not tested in other applications.

Calculated MW	134 kDa. ( Note )
Specificity/Sensitivity	The full-length EGFR includes the 24 aa signal peptide that is cleaved during EGFR maturation. The Tyr1173 of mature EGFR is equivalent to Tyr1197 of uncleaved EGFR.

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PROPERTIES

Form	Liquid
Buffer	PBS, 15mM sodium azide
Storage	Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC. DO NOT FREEZE.
Concentration	1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species	Human
Immunogen	Synthetic phospho-peptide covering sequence around tyrosine 1173 of human EGFR.
Purification	Protein A purified
Conjugation	Unconjugated
Note	For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

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TARGET

Synonyms	EGFR,ERBB,ERBB1,HER1,NISBD2,PIG61,epidermal growth factor receptor,mENA,EGF Receptor
Cellular Localization	Cell membrane,Endoplasmic reticulum membrane,Golgi apparatus membrane,Nucleus membrane,Endosome,Endosome membrane,Nucleus,Secreted
Background	The protein encoded by this gene is a transmembrane glycoprotein that is a member of the protein kinase superfamily. This protein is a receptor for members of the epidermal growth factor family. EGFR is a cell surface protein that binds to epidermal growth factor. Binding of the protein to a ligand induces receptor dimerization and tyrosine autophosphorylation and leads to cell proliferation. Mutations in this gene are associated with lung cancer. [provided by RefSeq, Jun 2016]
Database	・ Gene ID: 1956 EGFR ・ UniProt: P00533 EGFR

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