抗Hsp90 beta抗体 | Anti-Hsp90 beta antibody

• 特長
• 価格
• DATA IMAGES
• 製品情報
• APPLICATION
• PROPERTIES
• TARGET

特長

• 高品質の抗体です。
• 幅広い研究分野に関連する抗体を取り揃えています。
• 使用されたアプリケーションや動物種などの情報が充実しています。
• 多数の論文で使用実績がある信頼性の高い抗体です。

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価格

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DATA IMAGES

	ICC/IF analysis of HeLa and NIH3T3 cells using GTX79247 HSP90 beta antibody. Green : Primary antibodyBlue : NucleiFixation : FormalinPermeabilization : 0.1% Triton X-100 in TBS for 10 minutesDilution : 1:50 for at least 1 hour at room temperature
	ICC/IF analysis of HepG2 cells using GTX79247 HSP90 beta antibody. Cells were probed without (right) or with(left) an antibody.Green : Primary antibodyBlue : NucleiRed : ActinFixation : formaldehydeDilution : 1:100 overnight at 4 ºC
	WB analysis of mouse HSP86 (Hsp90 alpha) or HSP84 (Hsp90 beta) and protein using GTX22928 Hsp90 alpha antibody (Lane 1) or GTX79247 HSP90 beta antibody (Lane 2).
	ICC/IF analysis of U251 cells using GTX79247 HSP90 beta antibody. Cells were probed without (right) or with(left) an antibody.Green : Primary antibodyBlue : NucleiRed : ActinFixation : formaldehydeDilution : 1:100 overnight at 4 ºC
	IP analysis of HeLa cell lysates using GTX79247 HSP90 beta antibody. IP reaction : 2μg antibody / 500μg lysateDilution : 1:1000
	ICC/IF analysis of A2058 Cells using GTX79247 HSP90 beta antibody. Cells were probed without (right) or with(left) an antibody.Green : Primary antibodyBlue : NucleiRed : ActinFixation : formaldehydeDilution : 1:100 overnight at 4 ºC
	FACS analysis of permeabilized, fixed HeLa cells using GTX79247 HSP90 beta antibody. Blue histogram : Primary antibody Black histogram : Isotype control antibodyDilution : 10 μg/ml incubated for 1 hour on ice
	IHC-P analysis of human tonsil tissue using GTX79247 HSP90 beta antibody. Left : Primary antibodyRight : Negative control without primary antibodyAntigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutesDilution : 1:100
	IHC-P analysis of human placenta tissue using GTX79247 HSP90 beta antibody. Left : Primary antibodyRight : Negative control without primary antibodyAntigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutesDilution : 1:20
	WB analysis of 50μg of the indicated whole cell lysates per well using GTX79247 HSP90 beta antibody. Dilution : 1:1000
	IHC-P analysis of human breast carcinoma tissue using GTX79247 HSP90 beta antibody. Left : Primary antibodyRight : Negative control without primary antibodyAntigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutesDilution : 1:100

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製品情報

Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Application	WB, ICC/IF, IHC-P, IHC-Fr, FACS, IP, IHC
Reactivity	Human, Mouse, Rat, Primate

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APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Dilution
WB	1:1000 - 1:20000
ICC/IF	10-20 μg/ml
IHC-P	Assay dependent
IHC-Fr	10 μg/ml
FACS	1-20 μg/ml
IP	2 μg
IHC	Assay dependent

Not tested in other applications.

Calculated MW	83 kDa. ( Note )
Specificity/Sensitivity	This antibody does not detect HSP86. The antibody is capable of precipitating HSP84 that is complexed with other proteins such as the aryl hydrocarbon (Ah) receptor.

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PROPERTIES

Form	Liquid
Buffer	Buffer, 1mg/ml BSA, 0.05% sodium azide
Storage	Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration	1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species	Mouse
Immunogen	Synthetic peptide corresponding to residues P(2) E E V H H G E E E V E(13) of mouse HSP84.
Purification	Purified by antigen-affinity chromatography
Conjugation	Unconjugated
Note	For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

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TARGET

Synonyms	heat shock protein 90 alpha (cytosolic), class B member 1 , 90kDa , AL022974 , C81438 , Hsp84 , Hsp84-1 , Hsp90 , Hspcb
Cellular Localization	Cytoplasm,Nucleus,Secreted,Cell membrane
Background	Heat shock proteins (HSP) are expressed in response to various biological stresses, including heat. HSP90 is a 90 kDa protein that is induced under stress conditions, but is also one of the most abundant cellular proteins found under non-stress conditions. HSP90 has been found to be associated with a number of other intracellular proteins, including steroid receptors, actin, tubulin, aryl hydrocarbon (Ah) receptor, and some kinases. Studies have shown that murine HSP90 exists as two forms, HSP84 and HSP86, coded by related but separate genes, with 86% amino acid sequence conservation. These forms are analogous to the two forms of human HSP90, HSP89 alpha and HSP89 beta. In an unstressed mouse fibroblast, the basal level of HSP84 is found to be double that of HSP86. However, after heat shock, HSP86 shows a greater increase. Studies also suggest that upon cellular differentiation, the level of HSP86, but not HSP84, decreases. HSP84 and HSP86, which may be subject to estrogenic regulation, have been found as components of the non-DNA binding form of mouse glucocorticoid receptor, but dissociated from the transformed DNA-binding form.
Database	・ Gene ID: 15516 Hsp90ab1 ・ UniProt: P11499 Hsp90ab1

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