抗p53 [DO-7]抗体 | Anti-p53 [DO-7] antibody

• 特長
• 価格
• DATA IMAGES
• 製品情報
• APPLICATION
• PROPERTIES
• TARGET

特長

• 高品質の抗体です。
• 幅広い研究分野に関連する抗体を取り揃えています。
• 使用されたアプリケーションや動物種などの情報が充実しています。
• 多数の論文で使用実績がある信頼性の高い抗体です。

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価格

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DATA IMAGES

	WB analysis of HEK293 cell lysate using GTX75255 p53 antibody [DO-7].
	IHC-P analysis of human breast carcinoma tissue using GTX75255 p53 antibody [DO-7].

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製品情報

Host	Mouse
Clonality	Monoclonal
Clone Name	DO-7
Isotype	IgG2b
Application	WB, IHC-P, IHC-Fr, FACS, IP
Reactivity	Human, Bovine

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APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Dilution
WB	1/1,000
IHC-P	1/1,000
IHC-Fr	Assay dependent
FACS	1/50-1/100
IP	Assay dependent
Note : IHC-P This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. Sodium citrate buffer pH 6.0 is recommended for this purpose. FACS Membrane permeabilisation is required for this application. Use 10μl of the suggested working dilution to label 106 cells in 100μl.

Not tested in other applications.

Calculated MW	44 kDa. ( Note )
Specificity/Sensitivity	This antibody recognizes an epitope at the N-terminal end of p53 between amino acids 20-25, binding to both wild type and mutant forms.

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PROPERTIES

Form	Liquid
Buffer	PBS, 0.09% Sodium Azide
Storage	Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration	1.0 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species	Human
Immunogen	Recombinant human p53.
Purification	Protein G purified
Conjugation	Unconjugated
Note	For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

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TARGET

Synonyms	Tumor Protein P53 , Bcc7 , Lfs1 , P53 , Trp53 , Tp53
Background	This gene encodes a tumor suppressor protein containing transcriptional activation, DNA binding, and oligomerization domains. The encoded protein responds to diverse cellular stresses to regulate expression of target genes, thereby inducing cell cycle arrest, apoptosis, senescence, DNA repair, or changes in metabolism. Mutations in this gene are associated with a variety of human cancers, including hereditary cancers such as Li-Fraumeni syndrome. Alternative splicing of this gene and the use of alternate promoters result in multiple transcript variants and isoforms. Additional isoforms have also been shown to result from the use of alternate translation initiation codons (PMIDs: 12032546, 20937277). [provided by RefSeq, Feb 2013]
Database	・ Gene ID: 7157 ・ UniProt: P04637

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