抗CD86 [BU63]抗体 | Anti-CD86 [BU63] antibody

• 特長
• 価格
• 製品情報
• APPLICATION
• PROPERTIES
• TARGET
• REFERENCEE(抜粋)

特長

• 高品質の抗体です。
• 幅広い研究分野に関連する抗体を取り揃えています。
• 使用されたアプリケーションや動物種などの情報が充実しています。
• 多数の論文で使用実績がある信頼性の高い抗体です。

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価格

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製品情報

Host	Mouse
Clonality	Monoclonal
Clone Name	BU63
Isotype	IgG1
Application	WB, ICC/IF, IHC-Fr, FACS, IP, IHC
Reactivity	Human

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APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Dilution
WB	Assay dependent
ICC/IF	Assay dependent
IHC-Fr	1/20-1/100
FACS	1/20-1/100
IP	Assay dependent
IHC	Assay dependent
Note : FACS Use 10μl of the suggested working dilution to label 106 cells in 100μl.

Not tested in other applications.

Calculated MW	38 kDa. ( Note )
Specificity/Sensitivity	Domain depletion epitope mapping studies indicate that the binding site of Mouse anti Human CD86, clone Bu63 is located within the Ig-v-like domain of human CD86 (Jeanin et al. 1997).

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PROPERTIES

Form	Liquid
Buffer	PBS, 0.09% Sodium Azide
Storage	Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration	1.0 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species	Human
Immunogen	Human peripheral blood lymphocytes.
Purification	Protein A purified From tissue culture supernatant
Conjugation	Unconjugated
Note	For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

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TARGET

Synonyms	Cd86 Molecule , B7-2 , B7.2 , B70 , Cd28Lg2 , Lab72 , Cd86
Background	This gene encodes a type I membrane protein that is a member of the immunoglobulin superfamily. This protein is expressed by antigen-presenting cells, and it is the ligand for two proteins at the cell surface of T cells, CD28 antigen and cytotoxic T-lymphocyte-associated protein 4. Binding of this protein with CD28 antigen is a costimulatory signal for activation of the T-cell. Binding of this protein with cytotoxic T-lymphocyte-associated protein 4 negatively regulates T-cell activation and diminishes the immune response. Alternative splicing results in several transcript variants encoding different isoforms.[provided by RefSeq, May 2011]
Database	・ Gene ID: 942 ・ UniProt: P42081

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REFERENCEE(抜粋)

Application Reference	Application/Reactivity
Carbone F et al. Int J Cardiol 2018; (Epub) Serum levels of osteopontin predict major adverse cardiovascular events in patients with severe carotid artery stenosis.	Application : IHC Reactivity : Human
Wang LT et al. Cancer Res 2017; (Epub) Intestine-specific homeobox gene ISX integrates interleukin-6 signaling, tryptophan catabolism and immune suppression.	Application : WB Reactivity : Human
McCarthy NE et al. J Immunol 2013; 191 (5):2752-63 Proinflammatory Vδ2+ T cells populate the human intestinal mucosa and enhance IFN-γ production by colonic αβ T cells.
Silk KM et al. J Biomed Biotechnol 2012; 2012:172420 Rapamycin conditioning of dendritic cells differentiated from human ES cells promotes a tolerogenic phenotype.	Application : FACS Reactivity : Human
Hovden AO et al. BMC Immunol 2011; 12:2 Maturation of monocyte derived dendritic cells with OK432 boosts IL-12p70 secretion and conveys strong T-cell responses.	Application : FACS

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