抗IL1 beta抗体 | Anti-IL1 beta antibody

• 特長
• 価格
• DATA IMAGES
• 製品情報
• APPLICATION
• PROPERTIES
• TARGET
• REFERENCEE(抜粋)

特長

• 高品質の抗体です。
• 幅広い研究分野に関連する抗体を取り揃えています。
• 使用されたアプリケーションや動物種などの情報が充実しています。
• 多数の論文で使用実績がある信頼性の高い抗体です。

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価格

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DATA IMAGES

	IL1 beta antibody detects IL1 beta protein at microglia by immunohistochemical analysis.Sample: Paraffin-embedded rat cerebellum.Green: IL1 beta stained by IL1 beta antibody (GTX74034) diluted at 1:250.Red: NF-H, a neuron marker, stained by NF-H antibody [GT114] (GTX634289) diluted at 1:500.Blue: Fluoroshield with DAPI (GTX30920).Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
	IL1 beta antibody detects IL1 beta protein by immunofluorescent analysis.Sample: DIV9 rat E18 primary cortical neuron and glia cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: IL1 beta stained by IL1 beta antibody (GTX74034) diluted at 1:500.Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [GT11710] (GTX631836) diluted at 1:500.Blue: Fluoroshield with DAPI (GTX30920).
	Untreated (–) and treated (+) THP-1 whole cell extract and conditioned medium (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with IL1 beta antibody (GTX74034) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
	An ELISA plate is coated with 50 μL of IL1 beta recombinant protein at concentration of 5 μg/mL. The coated protein is detected with anti-IL1 beta antibody (GTX74034) at concentration ranged from 0.01 to 0.15 μg/mL.
	Raw264.7 whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with IL1 beta antibody (GTX74034) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
	Untreated (–) and treated (+) Raw264.7 whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with IL1 beta antibody (GTX132080) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
	Untreated (–) and treated (+) Raw264.7 whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with IL1 beta antibody (GTX74034) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
	IL1 beta antibody detects IL1 beta protein at microglia by immunohistochemical analysis.Sample: Frozen-sectioned mouse hippocampus.Green: IL1 beta stained by IL1 beta antibody (GTX74034) diluted at 1:250.Blue: Fluoroshield with DAPI (GTX30920).

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製品情報

Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Application	WB, ICC/IF, IHC-P, IHC-Fr, ELISA, Functional Assay, IHC
Reactivity	Human, Mouse, Rat

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APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Dilution
WB	1:500-1:3000
ICC/IF	1:100-1:1000
IHC-P	1:100-1:1000
IHC-Fr	1:100-1:1000
ELISA	0.5-2μg/ml
Functional Assay	Assay dependent
IHC	Assay dependent

Not tested in other applications.

Calculated MW	31 kDa. ( Note )
Positive Control	Raw264.7 (50 ng/ml LPS treatment for 4 hr) , *skin tissue
Specificity/Sensitivity	This antibody may be used in an indirect ELISA or as a capture reagent in a sandwich ELISA with GTX74033 as the detection reagent.

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PROPERTIES

Form	Liquid
Buffer	PBS
Storage	Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration	1.3 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species	Mouse
Immunogen	Recombinant murine IL-1 beta
Purification	Purified by antigen-affinity chromatography.
Conjugation	Unconjugated
Note	For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

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TARGET

Synonyms	interleukin 1 beta , IL-1beta , Il-1b
Database	・ Gene ID: 16176 Il1b ・ UniProt: P10749 Il1b

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REFERENCEE(抜粋)

Application Reference	Application/Reactivity
Mamantopoulos M et al. Front Immunol 2019; 10 : 2463 El Tor Biotype Vibrio cholerae Activates the Caspase-11-Independent Canonical Nlrp3 and Pyrin Inflammasomes.	Application : WB Reactivity : Mouse
Vande Walle L et al. PLoS Biol 2019; 17 (9) : e3000354 MCC950/CRID3 potently targets the NACHT domain of wild-type NLRP3 but not disease-associated mutants for inflammasome inhibition.	Application : WB Reactivity : Human
Wei L et al. Brain Res 2019; : 146423 Complement C3 participates in the function and mechanism of traumatic brain injury at simulated high altitude.	Application : IHC-P Reactivity : Rat
Reitz CJ et al. Commun Biol 2019; 2 : 353 SR9009 administered for one day after myocardial ischemia-reperfusion prevents heart failure in mice by targeting the cardiac inflammasome.	Application : WB Reactivity : Mouse
Lieselotte Vande Walle et al. bioRxiv 2019; (Epub) MCC950/CRID3 potently targets the NACHT domain of wildtype NLRP3 but not disease-associated mutants for inflammasome inhibition.	Application : WB Reactivity : Mouse

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