抗alpha Smooth Muscle Actin [1A4] (FITC)抗体 | Anti-alpha Smooth Muscle Actin [1A4] (FITC) antibody

• 特長
• 価格
• 製品情報
• APPLICATION
• PROPERTIES
• TARGET
• REFERENCEE(抜粋)

特長

• 高品質の抗体です。
• 幅広い研究分野に関連する抗体を取り揃えています。
• 使用されたアプリケーションや動物種などの情報が充実しています。
• 多数の論文で使用実績がある信頼性の高い抗体です。

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価格

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製品情報

Host	Mouse
Clonality	Monoclonal
Clone Name	1A4
Isotype	IgG2a
Application	ICC/IF, IHC-Fr, FACS
Reactivity	Human, Mouse, Rat, Rabbit, Guinea pig, Baboon

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APPLICATION

Application Note

For IF: Use at 10-20 μg/ml for 2 hours at room temperature. For IF: paraffin-embedded or frozen sections, as well as on cell smears can be used. Optimal dilutions/concentrations should be determined by the end user.

Calculated MW	42 kDa. ( Note )
Positive Control	Bowel
Specificity/Sensitivity	This antibody reacts with the alpha-smooth muscle isoform of actin. It does not react with actin from fibroblasts (beta- and gamma-cytoplasmic), striated muscle (alpha-sarcomeric) and myocardium (alpha-myocardial).

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PROPERTIES

Form	Liquid
Buffer	50 mM potassium phosphate, 150 mM Sodium Chloride, 10mg/ml BSA, carrier polysaccharides, and 0.05% Sodium Azide, pH 7.2.
Storage	Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC. DO NOT FREEZE. Protect from light.
Antigen Species	Human
Immunogen	The N-terminal decapeptide of alpha-smooth muscle actin
Purification	Unpurified
Conjugation	Fluorescein isothiocyanate (FITC) _x000B_Absorption : 495 nm_x000B_ ; Emission : 528 nm._x000B_ Ratio : 0.7 molecules FITC per Mouse IgG molecule.
Note	For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

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TARGET

Synonyms	Actin, Alpha 2, Smooth Muscle, Aorta , Actsa , Acta2
Cellular Localization	Cytoplasmic
Background	The protein encoded by this gene belongs to the actin family of proteins, which are highly conserved proteins that play a role in cell motility, structure and integrity. Alpha, beta and gamma actin isoforms have been identified, with alpha actins being a major constituent of the contractile apparatus, while beta and gamma actins are involved in the regulation of cell motility. This actin is an alpha actin that is found in skeletal muscle. Defects in this gene cause aortic aneurysm familial thoracic type 6. Multiple alternatively spliced variants, encoding the same protein, have been identified. [provided by RefSeq, Nov 2008]
Database	・ Gene ID: 59 ・ UniProt: P62736

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REFERENCEE(抜粋)

Application Reference	Application/Reactivity
Aslani F et al. Hum Reprod 2015; 30 (2) : 417-31 Targeting high mobility group box protein 1 ameliorates testicular inflammation in experimental autoimmune orchitis.
Akishima-Fukasawa Y et al. Am J Clin Pathol 2010; 134 (1) : 26177 Significance of PGP9.5 expression in cancer-associated fibroblasts for prognosis of colorectal carcinoma.
Backhouse S et al. Invest Ophthalmol Vis Sci 2010; 51 (12) : 6162-71 Effect of induced myopia on scleral myofibroblasts and in vivo ocular biomechanical compliance in the guinea pig.	Application : IHC-Fr Reactivity : Guinea pig
Gorchs L et al. Front Oncol 2015; 5:87 Cancer-Associated Fibroblasts from Lung Tumors Maintain Their Immunosuppressive Abilities after High-Dose Irradiation.	Application : FACS Reactivity : Human
Wan W et al. Cardiovasc Res 2015; 106 (3):478-87 Atypical chemokine receptor 1 deficiency reduces atherogenesis in ApoE-knockout mice.	Application : IHC-Fr Reactivity : Mouse

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