抗Cytokeratin 20 [Ks20.8]抗体 | Anti-Cytokeratin 20 [Ks20.8] antibody

• 特長
• 価格
• 製品情報
• APPLICATION
• PROPERTIES
• TARGET
• REFERENCEE(抜粋)

特長

• 高品質の抗体です。
• 幅広い研究分野に関連する抗体を取り揃えています。
• 使用されたアプリケーションや動物種などの情報が充実しています。
• 多数の論文で使用実績がある信頼性の高い抗体です。

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価格

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製品情報

Host	Mouse
Clonality	Monoclonal
Clone Name	Ks20.8
Isotype	IgG2a
Application	ICC/IF, IHC-P, IHC (Formalin)
Reactivity	Human, Mouse

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APPLICATION

Application Note

Used to identify and differentiate different carcinomas. This antibody can be used on formalin-fixed, paraffin-embedded tissue sections. Prolonged fixation in buffered formalin can destroy the epitope. For optimal results, tissue sections should either be treated with high-temperature based antigenic unmasking technique, or with proteolytic enzymes such as AutoZyme or Pronase. The optimal conditions should be determined by the individual laboratory. This antibody is not suitable for use on cryostat sections or cell smears due to non-specific cross-reaction with negative epithelia. Dilute according to the particular application being used. In general, the 0.05M Borate pH 8.0 containing 0.15M Sodium Chloride, 0.05% Sodium Azide, is a good diluent to use with most antibodies.

Calculated MW	48 kDa. ( Note )
Positive Control	Human Normal Colon, Colon Carcinoma
Specificity/Sensitivity	This antibody reacts with the 46 kD cytokeratin polypeptide 20, a type I cytokeratin that is found in duodenal epithelium, glandular gastric and pyloric epithelium, urothelium and Merkel cells of the epidermis. It does not cross-react with other cytokeratin polypeptides or other intermediate filaments. This cytokeratin is expressed in colon carcinoma, and to a lesser degree in gastric carcinoma. Other positive carcinomas include gall bladder, bile duct, ductal cells of pancreas, mucinous ovarian tumors and transitional cell carcinomas. Carcinoma of breast, lung, endometrium and non-mucinous tumors of ovary are not recognized byThis antibody. Merkel cell carcinomas of the skin show strong reactivity withThis antibody.

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PROPERTIES

Form	Liquid
Buffer	1X PBS buffer with 0.1% sodium azide.
Storage	Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC. DO NOT FREEZE.
Concentration	Batch dependent (Please refer to the vial label for the specific concentration.)
Antigen Species	Human
Immunogen	Cytokeratin preparation of villi of human duodenal mucosa.
Conjugation	Unconjugated
Note	For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

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TARGET

Synonyms	Keratin 20 , Cd20 , Ck-20 , Ck20 , K20 , Krt21 , Krt20
Background	Monoclonal Mouse Anti-Human Cytokeratin 20
Database	・ Gene ID: 54474 ・ UniProt: P35900

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REFERENCEE(抜粋)

Application Reference	Application/Reactivity
Noguchi A et al. Oncol Rep 2013; 30 (6) : 256979 Pulmonary tumors associated with the JC virus T-antigen in a transgenic mouse model.	Application : IHC-P Reactivity : Mouse
Lin SP et al. PLoS One 2012; 7 (11) : e49605 Survival of cancer stem cells under hypoxia and serum depletion via decrease in PP2A activity and activation of p38-MAPKAPK2-Hsp27.	Application : ICC/IF
Wang JY et al. J Cell Biochem 2012; 113 (5) : 1733-43 Silibinin suppresses the maintenance of colorectal cancer stem-like cells by inhibiting PP2A/AKT/mTOR pathways.	Application : ICC/IF Reactivity : Human

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