抗MDM2 [SMP14]抗体 | Anti-MDM2 [SMP14] antibody

• 特長
• 価格
• DATA IMAGES
• 製品情報
• APPLICATION
• PROPERTIES
• TARGET

特長

• 高品質の抗体です。
• 幅広い研究分野に関連する抗体を取り揃えています。
• 使用されたアプリケーションや動物種などの情報が充実しています。
• 多数の論文で使用実績がある信頼性の高い抗体です。

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価格

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DATA IMAGES

Untreated (–) and treated (+) HepG2 whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with MDM2 antibody (GTX70278) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.Multiple bands were observed 60 ~ 97 kDa. It is possibly due to alternative splicing.Reference:PMID: 9840926

IHC-P analysis of human breast carcinoma tissue using GTX70278 MDM2 antibody [SMP14].

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製品情報

Host	Mouse
Clonality	Monoclonal
Clone Name	SMP14
Isotype	IgG1
Application	WB, IHC-P, IHC-Fr, IP, IHC (Resin sections)
Reactivity	Human, Mouse

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APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Dilution
WB	1:500-1:3000
IHC-P	1/200-1/500
IHC-Fr	Assay dependent
IP	Assay dependent
IHC (Resin sections)	Assay dependent
Note : IHC-P This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. EDTA pH8.0 is recommended for this purpose.

Not tested in other applications.

Calculated MW	55 kDa. ( Note )
Positive Control	HepG2 , HepG2 (starvation for 16 hr, and 1 μM Staurosporine treatment for 24 hr) ,

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PROPERTIES

Form	Liquid
Buffer	PBS, 0.09% Sodium Azide
Storage	Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration	1.0 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species	Human
Immunogen	Synthetic peptide, CSRPSTSSRRRAISE, corresponding to amino acids 154-167 of human MDM2
Purification	Protein G purified
Conjugation	Unconjugated
Note	For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

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TARGET

Synonyms	Mdm2 Proto-Oncogene , Actfs , Hdmx , Hdm2 , Mdm2
Background	This gene encodes a nuclear-localized E3 ubiquitin ligase. The encoded protein can promote tumor formation by targeting tumor suppressor proteins, such as p53, for proteasomal degradation. This gene is itself transcriptionally-regulated by p53. Overexpression or amplification of this locus is detected in a variety of different cancers. There is a pseudogene for this gene on chromosome 2. Alternative splicing results in a multitude of transcript variants, many of which may be expressed only in tumor cells. [provided by RefSeq, Jun 2013]
Database	・ Gene ID: 4193 ・ UniProt: Q00987

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