抗Estrogen Receptor beta [14C8]抗体 | Anti-Estrogen Receptor beta [14C8] antibody

• 特長
• 価格
• DATA IMAGES
• 製品情報
• APPLICATION
• PROPERTIES
• TARGET
• REFERENCE

特長

• 高品質の抗体です。
• 幅広い研究分野に関連する抗体を取り揃えています。
• 使用されたアプリケーションや動物種などの情報が充実しています。
• 多数の論文で使用実績がある信頼性の高い抗体です。

目次に戻る

価格

目次に戻る

DATA IMAGES

	Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Estrogen Receptor beta antibody [14C8] (GTX70174) diluted at 1:5000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
	Various tissue extracts (20 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Estrogen Receptor beta antibody [14C8] (GTX70174) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
	B. Infiltrating lobular carcinoma of the breast.
	The WB analysis of Estrogen Receptor beta antibody [14C8] was published by Thomas C and colleagues in the journal Breast Cancer Res in 2012 . PMID: 23158001
	The WB analysis of Estrogen Receptor beta antibody [14C8] was published by Thomas C and colleagues in the journal Breast Cancer Res in 2012 . PMID: 23158001
	The WB analysis of Estrogen Receptor beta antibody [14C8] was published by Thomas C and colleagues in the journal Breast Cancer Res in 2012 . PMID: 23158001
	The IHC-P analysis of Estrogen Receptor beta antibody [14C8] was published by Samartzis N and colleagues in the journal Reprod Biol Endocrinol in 2012.PMID: 22520060
	Estrogen Receptor beta antibody [14C8] detects Estrogen Receptor beta protein at nucleus by immunohistochemical analysis.Sample: Paraffin-embedded human breast carcinoma.Estrogen Receptor beta stained by Estrogen Receptor beta antibody [14C8] (GTX70174) diluted at 1:200.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
	The WB analysis of Estrogen Receptor beta antibody [14C8] was published by Thomas C and colleagues in the journal Breast Cancer Res in 2012 . PMID: 23158001
	The WB analysis of Estrogen Receptor beta antibody [14C8] was published by Thomas C and colleagues in the journal Breast Cancer Res in 2012 . PMID: 23158001

目次に戻る

製品情報

Host	Mouse
Clonality	Monoclonal
Clone Name	14C8
Isotype	IgG2b
Application	WB, ICC/IF, IHC-P, FACS, Dot, ChIP assay, IHC
Reactivity	Human, Mouse, Monkey

目次に戻る

APPLICATION

Application Note

For IHC, a recommended protocol is provided. For WB: Use at a concentration of 1-5 μg/ml. For ChIP: Use at an dependent assay. Optimal dilutions/concentrations should be determined by the researcher.

Calculated MW	58-60 kDa. ( Note )
Positive Control	Myc-DDK-tagged ESR2-transfected 293T
Specificity/Sensitivity	Strong Erb staining in epithelial cell nuclei. Occasional weak to moderate staining is seen in surrounding stromal and endothelial cell nuclei. Sporadic light cytoplasmic staining is sometimes observed. We have also successfully detected ERb in colon and ovarian tumors using the same antibody. Knockdown/Knockout validation was supported by references (PMID:27323858)

目次に戻る

PROPERTIES

Form	Liquid
Buffer	PBS
Storage	Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration	1.23 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species	Human
Immunogen	Amino acids 1-153 of human ER-beta expressed in E. coli.
Purification	Protein G purified
Conjugation	Unconjugated
Note	For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

目次に戻る

TARGET

Synonyms	Estrogen Receptor 2 , Er-Beta , Esr-Beta , Esrb , Estrb , Erb , Nr3A2 , Esr2
Background	The human ER-beta is a newly discovered estrogen receptor initially cloned and characterized from testis. The size and structure of ER-beta is very similar to ER-alpha with the ligand and DNA binding domains being highly conserved, while the amino terminus which serves as their transactivation domain has diverged significantly. Similar in function to ER-alpha ER-beta binds to estrogen with a high affinity and regulates estrogen specific gene activation through direct interaction with estrogen response elements (ERE's).
Database	・ Gene ID: 2100 ・ UniProt: Q92731

目次に戻る

REFERENCE

Application Reference	Application/Reactivity
Asavasupreechar T et al. J Steroid Biochem Mol Biol 2019; 193 : 105440 Sex steroid metabolism and actions in non-small cell lung carcinoma.
Fan W et al. Biochem Biophys Res Commun 2019; 511 (2) : 468-475 Estrogen receptors participate in carcinogenesis signaling pathways by directly regulating NOD-like receptors.	Application : ChIP assay Reactivity : Human
Kumata H et al. Hepatol Res 2018; (Epub) Steroidogenesis in ovarian-like mesenchymal stroma of hepatic and pancreatic mucinous cystic neoplasms.
Andersson S et al. Nat Commun 2017; 8 : 15840 Insufficient antibody validation challenges oestrogen receptor beta research.	Application : WB Reactivity : Human
Rupp NJ et al. Breast J 2017; (Epub) Solid papillary carcinoma of the breast with an associated Cytokeratin 7- negative Paget's disease of the nipple. Report of a first case.	Application : IHC-P Reactivity : Human

目次に戻る