抗Hec1 (phospho Ser 55)抗体 | Anti-Hec1 (phospho Ser 55) antibody

• 特長
• 価格
• DATA IMAGES
• 製品情報
• APPLICATION
• PROPERTIES
• TARGET
• REFERENCEE(抜粋)

特長

• 高品質の抗体です。
• 幅広い研究分野に関連する抗体を取り揃えています。
• 使用されたアプリケーションや動物種などの情報が充実しています。
• 多数の論文で使用実績がある信頼性の高い抗体です。

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価格

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DATA IMAGES

	Immunofluorescent staining of Hec1 phospho Ser55 in cells treated with Hec1 siRNA (bottom) or control siRNA (top)
	Purified 6his-Hec1 was subject to a kinase assay with recombinant Aurora B and gamma-p32-labelled ATP. The resultant phospho-Hec1 were detected by measuring radioactive signal and by WB analysis with pSer55 antibody
	GFP-tagged Hec1 wild type, S55A or 6A (Ser55 and five other Ser/Thr residues changed to Ala) mutant was ectopically expressed in HeLa cells and the cell lysate was used for WB analysis with the Hec1 pSer55 antibody (diluted at 1:500)
	Untreated (–) and treated (+) HeLa whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Hec1 (phospho Ser 55) antibody (GTX70017) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

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製品情報

Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Application	WB, ICC/IF, IP, ELISA
Reactivity	Human, Mouse, Rat, Kangaroo rat

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APPLICATION

Application Note

Recommended Starting Dilutions:
For WB: 1:500-1:3000
For IP: Use at a dilution of 2-4 μg/mL for 1 mg cell extract
For ICC/IF: Use at a dilution of 1:300
Not yet tested in other applications. Optimal dilutions should be determined experimentally by the researcher.

Calculated MW	80 kDa. ( Note )
Positive Control	HeLa , Blocking peptide (GTX70017-PEP) , HeLa (100 ng/ml Nocodazole treatment for 24 hr)

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PROPERTIES

Form	Liquid
Buffer	1XPBS, 1% BSA, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.
Storage	Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration	2.57 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species	Human
Immunogen	Synthetic phosphopeptide (GTX70017-PEP) corresponding to the amino acids around Ser 55 conjugated to KLH
Purification	Purified by antigen-affinity chromatography.
Conjugation	Unconjugated
Note	For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

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TARGET

Synonyms	NDC80 kinetochore complex component , HEC , HEC1 , HsHec1 , KNTC2 , TID3 , hsNDC80
Cellular Localization	Nucleus , Kinetochore
Background	HEC is one of several proteins involved in spindle checkpoint signaling. This surveillance mechanism assures correct segregation of chromosomes during cell division by detecting unaligned chromosomes and causing prometaphase arrest until the proper bipolar attachment of chromosomes is achieved.[supplied by OMIM]
Database	・ Gene ID: 10403 NDC80 ・ UniProt: O14777 NDC80

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REFERENCEE(抜粋)

Application Reference	Application/Reactivity
Smith RJ et al. Cell Rep 2019; 28 (8) : 2206-2219.e8 PP1 and PP2A Use Opposite Phospho-dependencies to Control Distinct Processes at the Kinetochore.	Application : ICC/IF Reactivity : Human
Asai Y et al. J Cell Biol 2019; 218 (10) : 3223-3236 Aurora B kinase activity is regulated by SET/TAF1 on Sgo2 at the inner centromere.	Application : WB Reactivity : Human
Taveras C et al. Cell Cycle 2019; 18 (12) : 1349-1363 A tension-independent mechanism reduces Aurora B-mediated phosphorylation upon microtubule capture by CENP-E at the kinetochore.	Application : ICC/IF Reactivity : Human
Richard J Smith et al. bioRxiv 2019; (Epub) PP1 and PP2A use opposite phospho-dependencies to control distinct processes at the kinetochore.
Nguyen AL et al. Curr Biol 2018; 28 (21) : 3458-3468.e5 Genetic Interactions between the Aurora Kinases Reveal New Requirements for AURKB and AURKC during Oocyte Meiosis.	Application : ICC/IF Reactivity : Human

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