抗Hec1 (phospho Ser 165)抗体 | Anti-Hec1 (phospho Ser 165) antibody

• 特長
• 価格
• DATA IMAGES
• 製品情報
• APPLICATION
• PROPERTIES
• TARGET
• REFERENCEE(抜粋)

特長

• 高品質の抗体です。
• 幅広い研究分野に関連する抗体を取り揃えています。
• 使用されたアプリケーションや動物種などの情報が充実しています。
• 多数の論文で使用実績がある信頼性の高い抗体です。

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価格

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DATA IMAGES

Mouse anti-Hec1 (9G3, cat# GTX 70268) and rabbit anti-Hec1 (phospho Ser165) antibodies were used to co-stain MCF10A cells. Insets show kinetochores of misaligned chromosomes. DAPI staining shows chromatin.

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製品情報

Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Application	WB, ICC/IF, ELISA
Reactivity	Human, Mouse, Rat, Kangaroo rat

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APPLICATION

Application Note

Recommended Starting Dilutions:
For WB: Use at a dilution of 1:300
For ICC/IF: Use at a dilution of 1:150
Not yet tested in other applications. Optimal dilutions should be determined experimentally by the researcher.

Calculated MW	80 kDa. ( Note )
Positive Control	HeLa , Blocking peptide (GTX70013-PEP)

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PROPERTIES

Form	Liquid
Buffer	1X PBS
Storage	Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration	0.127 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species	Human
Immunogen	Synthetic phosphopeptide (GTX70013-PEP) corresponding to the amino acids around Ser 165 conjugated to KLH
Purification	Affinity purified using antigen
Conjugation	Unconjugated
Note	For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

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TARGET

Synonyms	Ndc80, Kinetochore Complex Component , Hec , Hec1 , Hshec1 , Kntc2 , Tid3 , Hsndc80 , Ndc80
Cellular Localization	Nucleus , Kinetochore
Background	HEC is one of several proteins involved in spindle checkpoint signaling. This surveillance mechanism assures correct segregation of chromosomes during cell division by detecting unaligned chromosomes and causing prometaphase arrest until the proper bipolar attachment of chromosomes is achieved.[supplied by OMIM]
Database	・ Gene ID: 10403 ・ UniProt: O14777

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REFERENCEE(抜粋)

Application Reference	Application/Reactivity
Fang Y et al. Oncotarget 2016; 7 (48) : 79327-79341 Preclinical activity of MBM-5 in gastrointestinal cancer by inhibiting NEK2 kinase activity.	Application : WB Reactivity : Human
Wei R et al. Mol Biol Cell 2011; 22 (19) : 3584-94 Phosphorylation of the Ndc80 complex protein, HEC1, by Nek2 kinase modulates chromosome alignment and signaling of the spindle assembly checkpoint.	Application : ICC/IF Reactivity : Kangaroo rat

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