抗Histone H2A.XS139ph (phospho Ser139) [GT2311]抗体 | Anti-Histone H2A.XS139ph (phospho Ser139) [GT2311] antibody
掲載日情報:2020/04/13 現在Webページ番号:141525
GeneTex社の抗Histone H2A.XS139ph (phospho Ser139) [GT2311]抗体 | Anti-Histone H2A.XS139ph (phospho Ser139) [GT2311] antibodyです。
※ 本製品は研究用です。研究用以外には使用できません。
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特長
- 高品質の抗体です。
- 幅広い研究分野に関連する抗体を取り揃えています。
- 使用されたアプリケーションや動物種などの情報が充実しています。
- 多数の論文で使用実績がある信頼性の高い抗体です。
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価格
[在庫・価格 :2025年04月26日 08時35分現在]
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Anti-Histone H2A.XS139ph (phospho Ser139), Mouse-Mono(GT2311) |
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Anti-Histone H2A.XS139ph (pSer139), Phosphorylated, Mouse(GT2311) |
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[在庫・価格 :2025年04月26日 08時35分現在]
Anti-Histone H2A.XS139ph (phospho Ser139), Mouse-Mono(GT2311)
文献数: 18
- 商品コード:GTX628789
- メーカー:GNT
- 包装:25μl
- 価格:¥30,000
- 在庫:1個
- 納期:10日程度 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等:
説明文 | レビューあり。 別名:H2A histone family member X,H2A.X,H2A/X,H2AX クローン:GT2311 Genbank No: 3014 |
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別包装品 | 別包装品あり | ||||||
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保存条件 | -20℃ | 法規備考 | |||||
抗原種 | Human | 免疫動物 | Mouse | ||||
交差性 | Human/Mouse/Rat | 適用 | IC,IF,IHC,IP,Western Blot | ||||
標識 | Unlabeled | 性状 | Protein A/G Affinity Purified | ||||
吸収処理 | クラス | IgG | |||||
クロナリティ | Monoclonal | フォーマット | |||||
掲載カタログ |
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製品記事 | 抗ヒストン抗体/修飾ヒストン抗体 使いっきり抗体 |
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関連記事 | GeneTex社における抗体の品質管理 |
Anti-Histone H2A.XS139ph (pSer139), Phosphorylated, Mouse(GT2311)
文献数: 18
- 商品コード:GTX628789
- メーカー:GNT
- 包装:100μl
- 価格:¥85,000
- 在庫:1個
- 納期:10日程度 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等:
説明文 | レビューあり。 別名:H2A histone family member X,H2A.X,H2A/X,H2AX クローン:GT2311 Genbank No: 3014 |
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別包装品 | 別包装品あり | ||||||
法規制等 | |||||||
保存条件 | -20℃ | 法規備考 | |||||
抗原種 | Human | 免疫動物 | Mouse | ||||
交差性 | Human/Mouse/Rat | 適用 | IC,IF,IHC,IP,Western Blot | ||||
標識 | Unlabeled | 性状 | Protein A/G Affinity Purified | ||||
吸収処理 | クラス | IgG | |||||
クロナリティ | Monoclonal | フォーマット | |||||
掲載カタログ |
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製品記事 | 抗ヒストン抗体/修飾ヒストン抗体 |
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関連記事 | GeneTex社における抗体の品質管理 |
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DATA IMAGES
![]() | Histone H2A.X (phospho S139) antibody immunoprecipitates histone H2A.X (phospho S139) protein in IP experiments. IP Sample: 500 μg HCT116 with CPT 30 μM treatment 24 hr whole cell lysate/extract A. 30 μg HCT116 whole with CPT 30 uM treatment cell lysate/extract B. Control with 2 μg of preimmune mouse IgG C. Immunoprecipitation of histone H2A.X (phospho S139) protein by 2 μg histone H2A.X (phospho S139) antibody (GTX628789) 15% SDS-PAGE The immunoprecipitated histone H2A.X (phospho S139) protein was detected by Human histone H2A.X (phospho S139) antibody (GTX628789) diluted at 1:1000. EasyBlot anti-mouse IgG (GTX221667-01) was used as a secondary reagent. |
![]() | Histone H2A.XS139ph (phospho Ser139) antibody [GT2311] detects Histone H2A.XS139ph (phospho Ser139) protein at nucleus on mouse testis by immunohistochemical analysis. Sample: Paraffin-embedded mouse testis. Histone H2A.XS139ph (phospho Ser139) antibody [GT2311] (GTX628789) dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min |
![]() | Histone H2A.X (phospho Ser139) antibody detects H2AFX protein at nuclear by confocal immunofluorescent analysis. Sample: 10μM Cisplatin treated (right) or untreated (left) HeLa cells were fixed in 4% paraformaldehyde for 15 min. Red: H2A.X protein stained by Histone H2A.X (phospho Ser139) antibody (GTX628789) diluted at 1:500. Green: alpha Tubulin antibody (GTX102078) diluted at 1:1000. Blue: Hoechst 33342 staining. [Images captured by Olympus FV1000 Confocal Laser Scanning Microscope] |
![]() | Histone H2A.X antibody detects H2AFX protein at nuclear by immunofluorescent analysis. Sample: 10μM Cisplatin treated (right) or untreated (left) HeLa cells were fixed in 4% paraformaldehyde for 15 min. Green: H2AFX protein stained by Histone H2A.Xantibody (GTX628789) diluted at 1:500. Blue: Hoechst 33342 staining. |
![]() | ![]() Histone H2A.X (phospho S139) antibody [GT2311] detects H2AFX protein by western blot analysis. A. 30 μg NIH-3T3 whole cell lysate/extract (untreated) B. 30 μg NIH-3T3 whole cell lysate/extract (30μM cisplatin treatment for 24hr) 15% SDS-PAGE Histone H2A.X (phospho S139) antibody [GT2311] (GTX628789) dilution: 1:1000 The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody. |
![]() | ![]() Histone H2A.X (phospho S139) antibody [GT2311] detects Histone H2A.X (phospho S139) [GT2311] protein by western blot analysis. Un-treated (-) and treated (+, 30 μM Cisplatin treatment for 24 hrs) PC-12 whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with Histone H2A.X (phospho S139) antibody [GT2311] (GTX628789) diluted by 1:500. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody. |
![]() | ![]() Histone H2A.XS139ph (phospho Ser139) antibody detects Histone H2A.XS139ph (phospho Ser139) protein by western blot analysis. Un-treated (-) and treated (+, 50 J/m2 UV treatment) U2OS whole cell extracts (16 μg) were separated by 12%-15% SDS-PAGE, and the membrane was blotted with Histone H2A.XS139ph (phospho Ser139) antibody (GTX628789) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody. |
![]() | ![]() Histone H2A.X (phospho S139) antibody [GT2311] detects H2AFX protein by western blot analysis. A. 30 μg HCT116 whole cell lysate/extract (untreated) B. 30 μg HCT116 whole cell lysate/extract (30 μM cisplatin treatment for 24hr) 12% SDS-PAGE Histone H2A.X (phospho S139) antibody [GT2311] (GTX628789) dilution: 1:1000 The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody. |
![]() | ![]() The WB analysis of Histone H2A.XS139ph (phospho Ser139) antibody [GT2311] was published by En J and colleagues in the journal PLoS Negl Trop Dis in 2017.PMID: 28783752 |
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製品情報
Host | Mouse |
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Clonality | Monoclonal |
Clone Name | GT2311 |
Isotype | IgG1 |
Application | WB, ICC/IF, IHC-P, IP |
Reactivity | Human, Mouse, Rat |
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APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.Application | Dilution |
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WB | 1:500-1:3000 |
ICC/IF | 1:100-1:1000 |
IHC-P | 1:100-1:1000 |
IP | 1:100-1:500 |
Calculated MW | 15 kDa. ( Note ) |
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Positive Control | HCT116 (30μM cisplatin treatment for 24hr) , NIH-3T3 (30μM cisplatin treatment for 24hr) , PC-12 (30uM cisplatin treatment for 24h) , U2OS treated UV (50 J/m2) |
Predict Reactivity | Bovine, Rhesus Monkey(>80% identity) |
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PROPERTIES
Form | Liquid |
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Buffer | PBS |
Storage | Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
Concentration | 0.92 mg/ml (Please refer to the vial label for the specific concentration.) |
Antigen Species | Human |
Immunogen | Carrier-protein conjugated synthetic peptide corresponding to residues around human Histone H2A.XS139ph (phospho Ser139). The exact sequence is proprietary. |
Purification | Affinity purified by Protein G. |
Conjugation | Unconjugated |
Note | For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption. |
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TARGET
Synonyms | H2A histone family member X , H2A.X , H2A/X , H2AX |
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Cellular Localization | Nucleus |
Background | Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene encodes a member of the histone H2A family, and generates two transcripts through the use of the conserved stem-loop termination motif, and the polyA addition motif. [provided by RefSeq] |
Database | ・ Gene ID: 3014 H2AFX ・ UniProt: P16104 H2AFX |
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REFERENCE
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