抗Oct4 [GT735]抗体 | Anti-Oct4 [GT735] antibody

• 2025/10/01

  試薬 プレゼント

  [GNT]【数量限定】早いもの勝ち！ジェンタくんキーホルダープレゼントキャンペーン[～2025/10/31] 終了間近

  期間：2025/10/01 ～2025/10/31

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  GeneTex International Corporation（GNT）

• 特長
• 価格
• DATA IMAGES
• 製品情報
• APPLICATION
• PROPERTIES
• TARGET

特長

• 高品質の抗体です。
• 幅広い研究分野に関連する抗体を取り揃えています。
• 使用されたアプリケーションや動物種などの情報が充実しています。
• 多数の論文で使用実績がある信頼性の高い抗体です。

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価格

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DATA IMAGES

	Oct4 antibody immunoprecipitates Oct4 protein in IP experiments. IP Sample: cell lysate/extract of Oct4 gene transfected 293T cells A. Cell lysate/extract of transfected 293T cell B. Control with 2 μg of preimmune mouse IgG C. Immunoprecipitation of Oct4 by 2 μg of Oct4 antibody (GTX627423) 12% SDS-PAGE The immunoprecipitated Oct4 protein was detected by Oct4 antibody (GTX627423) diluted at 1:1000. EasyBlot anti-mouse IgG (GTX221667-01) was used as a secondary reagent.
	Sample (30 ug of whole cell lysate) A: NT2D1 10% SDS PAGE GTX627423 diluted at 1:1000
	Oct4 antibody [GT735] detects Oct4 protein by Western blot analysis.A. 30 μg human ESC whole cell lysate/extract10 % SDS-PAGE (GTX627423) dilution: 1:1000
	Oct4 antibody [GT735] detects POU5F protein by flow cytomertry analysis. Sample: Human embryonic stem cells Black: Isotype control dilution: 1:50 Green: Oct4 antibody [GT735] dilution: 1:50
	Sample (10 ug of whole cell lysate) A: Non-transfected HeLa lysates B: OCT 3/4 transfected HeLa lysates 10% SDS PAGE GTX627423 diluted at 1:5000
	Oct4 antibody detects Oct4 protein by immunofluorescent analysis. Sample: human embryonic stem cell were fixed in 4% paraformaldehyde for 15 min. Green: Oct4 protein stained by Oct4 antibody (GTX627423) diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 μm.

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製品情報

Host	Mouse
Clonality	Monoclonal
Clone Name	GT735
Isotype	IgG1
Application	WB, ICC/IF, FACS, IP
Reactivity	Human

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APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Dilution
WB	1:1000-1:10000
ICC/IF	1:100-1:1000
FACS	1:50-1:200
IP	1:100-1:500

Not tested in other applications.

Calculated MW	39 kDa. ( Note )
Positive Control	NT2D1 , human ESC , HeLa transfected recombinant OCT3/4 protein
Predict Reactivity	Rabbit, Bovine, Cat, Pig, Chimpanzee, Rhesus Monkey(>80% identity)

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PROPERTIES

Form	Liquid
Buffer	PBS
Storage	Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration	1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species	Human
Immunogen	Recombinant protein encompassing a sequence within the center region of human Oct4. The exact sequence is proprietary.
Purification	Affinity purified by Protein G.
Conjugation	Unconjugated
Note	For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

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TARGET

Synonyms	POU class 5 homeobox 1 , OCT3 , OCT4 , OTF-3 , OTF3 , OTF4 , Oct-3 , Oct-4
Cellular Localization	Nucleus
Background	This gene encodes a transcription factor containing a POU homeodomain. This transcription factor plays a role in embryonic development, especially during early embryogenesis, and it is necessary for embryonic stem cell pluripotency. A translocation of this gene with the Ewing's sarcoma gene, t(6;22)(p21;q12), has been linked to tumor formation. Alternative splicing, as well as usage of alternative translation initiation codons, results in multiple isoforms, one of which initiates at a non-AUG (CUG) start codon. Related pseudogenes have been identified on chromosomes 1, 3, 8, 10, and 12. [provided by RefSeq]
Database	・ Gene ID: 5460 POU5F1 ・ UniProt: Q01860 POU5F1

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