抗CDKN2A / p16INK4a [1D7D2]抗体 | Anti-CDKN2A / p16INK4a [1D7D2] antibody

• 特長
• 価格
• DATA IMAGES
• 製品情報
• APPLICATION
• PROPERTIES
• TARGET

特長

• 高品質の抗体です。
• 幅広い研究分野に関連する抗体を取り揃えています。
• 使用されたアプリケーションや動物種などの情報が充実しています。
• 多数の論文で使用実績がある信頼性の高い抗体です。

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価格

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DATA IMAGES

	FACS analysis of HEK293 cells using GTX60663 CDKN2A / p16INK4a antibody [1D7D2].Green : CDKN2A / p16INK4a Red : negative control
	IHC-P analysis of endometrial cancer tissue using GTX60663 CDKN2A / p16INK4a antibody [1D7D2].
	IHC-P analysis of lung cancer tissue using GTX60663 CDKN2A / p16INK4a antibody [1D7D2].
	ELISA analysis of antigen using GTX60663 CDKN2A / p16INK4a antibody [1D7D2]. Black : Control antigen 100ng Purple : Antigen 10ng Blue : Antigen 50ng Red : Antigen 100ng
	WB analysis of HEK293 (1) and CDKN2A (AA: 1-156)-hIgGFc transfected HEK293 (2) cell lysate using GTX60663 CDKN2A / p16INK4a antibody [1D7D2].

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製品情報

Host	Mouse
Clonality	Monoclonal
Clone Name	1D7D2
Isotype	IgG1
Application	WB, IHC-P, FACS, ELISA
Reactivity	Human

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APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Dilution
WB	1/500 - 1/2000
IHC-P	1/200 - 1/1000
FACS	1/200 - 1/400
ELISA	1/10000

Not tested in other applications.

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PROPERTIES

Form	Liquid
Buffer	Ascites, 0.03% sodium azide
Storage	Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Antigen Species	Human
Immunogen	Purified recombinant fragment of human CDKN2A (AA: 1-156) expressed in E. Coli.
Purification	Unpurified
Conjugation	Unconjugated
Note	For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

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TARGET

Synonyms	cyclin dependent kinase inhibitor 2A , ARF , CDK4I , CDKN2 , CMM2 , INK4 , INK4A , MLM , MTS-1 , MTS1 , P14 , P14ARF , P16 , P16-INK4A , P16INK4 , P16INK4A , P19 , P19ARF , TP16
Cellular Localization	Cytoplasm,Nucleus,nucleoplasm,Nucleolus
Background	This gene generates several transcript variants which differ in their first exons. At least three alternatively spliced variants encoding distinct proteins have been reported, two of which encode structurally related isoforms known to function as inhibitors of CDK4 kinase. The remaining transcript includes an alternate first exon located 20 Kb upstream of the remainder of the gene; this transcript contains an alternate open reading frame (ARF) that specifies a protein which is structurally unrelated to the products of the other variants. This ARF product functions as a stabilizer of the tumor suppressor protein p53 as it can interact with, and sequester, the E3 ubiquitin-protein ligase MDM2, a protein responsible for the degradation of p53. In spite of the structural and functional differences, the CDK inhibitor isoforms and the ARF product encoded by this gene, through the regulatory roles of CDK4 and p53 in cell cycle G1 progression, share a common functionality in cell cycle G1 control. This gene is frequently mutated or deleted in a wide variety of tumors, and is known to be an important tumor suppressor gene. [provided by RefSeq, Sep 2012]
Database	・ Gene ID: 1029 CDKN2A ・ UniProt: P42771 ・ UniProt: Q8N726

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