抗Caspase 8 [1H11]抗体 | Anti-Caspase 8 [1H11] antibody

• 特長
• 価格
• DATA IMAGES
• 製品情報
• APPLICATION
• PROPERTIES
• TARGET

特長

• 高品質の抗体です。
• 幅広い研究分野に関連する抗体を取り揃えています。
• 使用されたアプリケーションや動物種などの情報が充実しています。
• 多数の論文で使用実績がある信頼性の高い抗体です。

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価格

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DATA IMAGES

	FACS analysis of NIH3T3 cells using GTX60510 Caspase 8 antibody [1H11].Blue : Caspase 8 Red : negative control
	IHC-P analysis of colon cancer tissue using GTX60510 Caspase 8 antibody [1H11].
	IHC-P analysis of liver cancer tissue using GTX60510 Caspase 8 antibody [1H11].
	ELISA analysis of antigen using GTX60510 Caspase 8 antibody [1H11]. Red : Control antigen 100ng Purple : Antigen 10ng Green : Antigen 50ng Blue : Antigen 100ng
	WB analysis of HeLa (1), Jurkat (2), THP-1 (3), NIH3T3 (4), Cos7 (5) and PC-12 (6) cell lysate using GTX60510 Caspase 8 antibody [1H11].

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製品情報

Host	Mouse
Clonality	Monoclonal
Clone Name	1H11
Isotype	IgG1
Application	WB, IHC-P, FACS, ELISA
Reactivity	Human, Mouse, Rat, Monkey

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APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Dilution
WB	1/500 - 1/2000
IHC-P	1/200 - 1/1000
FACS	1/200 - 1/400
ELISA	1/10000

Not tested in other applications.

Calculated MW	55 kDa. ( Note )

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PROPERTIES

Form	Liquid
Buffer	Ascites, 0.03% sodium azide
Storage	Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Antigen Species	Human
Immunogen	Purified recombinant fragment of human CASP8(AA : 302-464) expressed in E. Coli.
Purification	Unpurified
Conjugation	Unconjugated
Note	For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

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TARGET

Synonyms	caspase 8 , ALPS2B , CAP4 , Casp-8 , FLICE , MACH , MCH5
Cellular Localization	Cytoplasm
Background	This gene encodes a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes composed of a prodomain, a large protease subunit, and a small protease subunit. Activation of caspases requires proteolytic processing at conserved internal aspartic residues to generate a heterodimeric enzyme consisting of the large and small subunits. This protein is involved in the programmed cell death induced by Fas and various apoptotic stimuli. The N-terminal FADD-like death effector domain of this protein suggests that it may interact with Fas-interacting protein FADD. This protein was detected in the insoluble fraction of the affected brain region from Huntington disease patients but not in those from normal controls, which implicated the role in neurodegenerative diseases. Many alternatively spliced transcript variants encoding different isoforms have been described, although not all variants have had their full-length sequences determined. [provided by RefSeq, Jul 2008]
Database	・ Gene ID: 841 CASP8 ・ UniProt: Q14790 CASP8

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