抗Histone H3K36me2 (Di-methyl Lys36) - ChIP grade抗体 | Anti-Histone H3K36me2 (Di-methyl Lys36) - ChIP grade antibody

• 特長
• 価格
• DATA IMAGES
• 製品情報
• APPLICATION
• PROPERTIES
• TARGET

特長

• 高品質の抗体です。
• 幅広い研究分野に関連する抗体を取り揃えています。
• 使用されたアプリケーションや動物種などの情報が充実しています。
• 多数の論文で使用実績がある信頼性の高い抗体です。

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価格

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DATA IMAGES

	ELISA was performed using a serial dilution of Histone H3K36me2 (di-Methyl Lys36) antibody in antigen coated wells. By plotting the absorbance against the antibody dilution, the titer of the antibody was estimated to be 1:31,000.
	ChIP was performed with HeLa nuclear extract and either 15 μl of H4K8ac antibody or 5 μg of control rabbit IgG. The precipitated DNA was detected by QPCR with primer set targeting to ALDOA promoter or MyoD.
	Dot blot analysis of peptides containing modified and unmodified sequences of histone H3 using H3K36me2 antibody at a dilution of 1:100,000. One hundred to 0.2 pmol of the peptide containing the respective histone modification were spotted on a membrane for analysis.
	ICC/IF analysis of HeLa cells using H3K36me2 antibody and DAPI. Cells were fixed with 4% formaldehyde for 10’ and blocked with PBS/TX-100 containing 5% normal goat serum and 1% BSA. The cells were immunofluorescently labelled with the H3K36me2 antibody at a dilution of 1:500 (green). The nuclei were stained with DAPI (blue).
	WB analysis of histone extracts (15 μg) from HeLa cells using H3K36me2 antibody diluted 1:1,000 in TBS-Tween containing 5% skimmed milk (lane 1). Lane 2 shows the same analysis after incubation of the antibody with 5 nmol blocking peptide for 1 hour at room temperature.

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製品情報

Host	Rabbit
Clonality	Polyclonal
Application	WB, ICC/IF, Dot, ELISA, ChIP assay, ChIP-seq
Reactivity	Human

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APPLICATION

Application Note

ChIP/ChIP-seq (5 μl/IP), ELISA (1:1,000), Dot blotting (1:100,000), Western blotting (1:1,000), Immunofluoresence (1:500)

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PROPERTIES

Form	Liquid
Buffer	Whole antiserum from rabbit containing 0.05% azide.
Storage	Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Purification	Whole antiserum from rabbit containing 0.05% azide.
Conjugation	Unconjugated
Note	For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

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TARGET

Synonyms

Histone H3 di Methyl Antibody, di Methyl Histone H3 Antibody, histone cluster 1, H3a Antibody, H3/A Antibody, HIST1H3A Antibody, Histone H3 di-Methyl Antibody, di-Methyl Lys36 Histone H3 Antibody, Histone H3 di-Methyl K36 Antibody, H3FA Antibody, di Methyl K36 Histone H3 Antibody, di Methyl Histone H3 Lys36 Antibody, diMethyl Histone H3 Antibody, diMethyl Lys36 Histone H3 Antibody, Histone H3 diMethyl Antibody, Methyl Histone H3 Antibody, di Methyl Histone H3 K36 Antibody, diMethyl K36 Histone H3 Antibody, Histone H3 di-Methyl Lys36 Antibody, di-Methyl Histone H3 Antibody, Histone H3 Methyl Antibody, di Methyl Lys36 Histone H3 Antibody, di-Methyl K36 Histone H3 Antibody

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