抗AMPK alpha (phospho Thr172)抗体 | Anti-AMPK alpha (phospho Thr172) antibody

• 特長
• 価格
• DATA IMAGES
• 製品情報
• APPLICATION
• PROPERTIES
• TARGET
• REFERENCEE(抜粋)

特長

• 高品質の抗体です。
• 幅広い研究分野に関連する抗体を取り揃えています。
• 使用されたアプリケーションや動物種などの情報が充実しています。
• 多数の論文で使用実績がある信頼性の高い抗体です。

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価格

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DATA IMAGES

	Untreated (–) and treated (+) MCF-7 whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with AMPK alpha (phospho Thr172) antibody (GTX52341) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
	Western blot analysis of AMPK alpha phosphorylation expression in Heatshock treated 293 whole cell lysates,The lane on the left is treated with the antigen-specific peptide.

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製品情報

Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Application	WB, IHC
Reactivity	Human, Mouse, Rat

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APPLICATION

Application Note

WB 1:500-1:2000, IHC 1:50-1:200

Calculated MW	64 kDa. ( Note )
Specificity/Sensitivity	AAPK1

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PROPERTIES

Form	Liquid
Buffer	phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage	Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration	1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species	Human
Immunogen	A synthesized peptide derived from human AMPK alpha around the phosphorylation site of Thr172
Purification	Antigen affinity purification
Conjugation	Unconjugated
Note	For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

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TARGET

Synonyms	Protein Kinase Amp-Activated Catalytic Subunit Alpha 1 , Ampk , Ampka1 , Prkaa1
Background	The protein encoded by this gene belongs to the ser/thr protein kinase family. It is the catalytic subunit of the 5'-prime-AMP-activated protein kinase (AMPK). AMPK is a cellular energy sensor conserved in all eukaryotic cells. The kinase activity of AMPK is activated by the stimuli that increase the cellular AMP/ATP ratio. AMPK regulates the activities of a number of key metabolic enzymes through phosphorylation. It protects cells from stresses that cause ATP depletion by switching off ATP-consuming biosynthetic pathways. Alternatively spliced transcript variants encoding distinct isoforms have been observed. [provided by RefSeq, Jul 2008]
Database	・ Gene ID: 5562 ・ UniProt: Q13131

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REFERENCEE(抜粋)

Application Reference	Application/Reactivity
Lu CC et al. Int J Oncol 2019; 54 (4) : 1271-1281 Metformin triggers the intrinsic apoptotic response in human AGS gastric adenocarcinoma cells by activating AMPK and suppressing mTOR/AKT signaling.
Yang Q et al. Nat Commun 2018; 9 (1) : 4667 PRKAA1/AMPKα1-driven glycolysis in endothelial cells exposed to disturbed flow protects against atherosclerosis.	Reactivity : Mouse
Yeh YT et al. Nutrients 2017; 9 (10) Chinese Olive (Canarium album L.) Fruit Extract Attenuates Metabolic Dysfunction in Diabetic Rats.	Application : WB Reactivity : Rat

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