抗EGFR (phospho Tyr1148)抗体 | Anti-EGFR (phospho Tyr1148) antibody

• 特長
• 価格
• DATA IMAGES
• 製品情報
• APPLICATION
• PROPERTIES
• TARGET

特長

• 高品質の抗体です。
• 幅広い研究分野に関連する抗体を取り揃えています。
• 使用されたアプリケーションや動物種などの情報が充実しています。
• 多数の論文で使用実績がある信頼性の高い抗体です。

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価格

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DATA IMAGES

	WB analysis of extracts from A431 cells untreated or treated with EGF using GTX50257 EGFR (phospho Tyr1148) antibody.
	IHC-P analysis of human breast carcinoma tissue using GTX50257 EGFR (phospho Tyr1148) antibody.Left : Primary antibodyRight : Primary antibody pre-incubated with the antigen specific peptide

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製品情報

Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Application	WB, IHC-P
Reactivity	Human

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APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Dilution
WB	1:500-1:1000
IHC-P	1:50-1:100

Not tested in other applications.

Calculated MW	134 kDa. ( Note )
Specificity/Sensitivity	The full-length EGFR includes the 24 aa signal peptide that is cleaved during EGFR maturation. The Tyr1148 of mature EGFR is equivalent to Tyr1172 of uncleaved EGFR.

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PROPERTIES

Form	Liquid
Buffer	PBS (without Mg²⁺ and Ca²⁺) pH 7.4, 150mM NaCl, 0.02% sodium azide, 50% glycerol
Storage	Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration	1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species	Human
Immunogen	Peptide sequence around phosphorylation site of tyrosine 1148 (P-D-Y(p)-Q-Q) derived from human EGFR.
Purification	Purified by antigen-affinity chromatography. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Conjugation	Unconjugated
Note	For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

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TARGET

Synonyms	epidermal growth factor receptor , ERBB , ERBB1 , HER1 , NISBD2 , PIG61 , mENA
Cellular Localization	Cell membrane,Endoplasmic reticulum membrane,Endosome,Endosome membrane,Nucleus
Background	The protein encoded by this gene is a transmembrane glycoprotein that is a member of the protein kinase superfamily. This protein is a receptor for members of the epidermal growth factor family. EGFR is a cell surface protein that binds to epidermal growth factor. Binding of the protein to a ligand induces receptor dimerization and tyrosine autophosphorylation and leads to cell proliferation. Mutations in this gene are associated with lung cancer. [provided by RefSeq, Jun 2016]
Database	・ Gene ID: 1956 EGFR ・ UniProt: P00533 EGFR

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