抗AKT (phospho Ser473)抗体 | Anti-AKT (phospho Ser473) antibody

• 特長
• 価格
• DATA IMAGES
• 製品情報
• APPLICATION
• PROPERTIES
• TARGET
• REFERENCEE(抜粋)

特長

• 高品質の抗体です。
• 幅広い研究分野に関連する抗体を取り揃えています。
• 使用されたアプリケーションや動物種などの情報が充実しています。
• 多数の論文で使用実績がある信頼性の高い抗体です。

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価格

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DATA IMAGES

	WB analysis of extracts from SK-BR-3 cells untreated, treated with insulin + EGF, U0126, or LY294002 using GTX50128 AKT (phospho Ser473) antibody.
	IHC-P analysis of human breast carcinoma tissue using GTX50128 AKT (phospho Ser473) antibody.Left : Primary antibodyRight : Primary antibody pre-incubated with the antigen specific peptide
	WB analysis of extracts from 293 cells treated with EGF or calf intestinal phosphatase (CIP) using GTX50128 AKT (phospho Ser473) antibody.
	IHC-P analysis of human lung carcinoma tissue using GTX50128 AKT (phospho Ser473) antibody.

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製品情報

Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Application	WB, IHC-P
Reactivity	Human, Mouse, Rat, Monkey

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APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Dilution
WB	1:500-1:1000
IHC-P	1:50-1:100

Not tested in other applications.

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PROPERTIES

Form	Liquid
Buffer	PBS (without Mg²⁺ and Ca²⁺) pH 7.4, 150mM NaCl, 0.02% sodium azide, 50% glycerol
Storage	Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration	1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species	Human
Immunogen	Peptide sequence around phosphorylation site of serine 473 (Q-F-S(p)-Y-S) derived from human AKT.
Purification	Purified by antigen-affinity chromatography. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Conjugation	Unconjugated
Note	For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

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TARGET

Synonyms	AKT serine/threonine kinase 1 , AKT serine/threonine kinase 2 , AKT serine/threonine kinase 3 , AKT , CWS6 , PKB , PKB-ALPHA , PRKBA , RAC , RAC-ALPHA , HIHGHH , PKBB , PKBBETA , PRKBB , RAC-BETA , MPPH , MPPH2 , PKB-GAMMA , PKBG , PRKBG , RAC-PK-gamma , RAC-gamma , STK-2
Cellular Localization	Cytoplasm,Nucleus,Cell membrane,Membrane
Database	・ Gene ID: 207 AKT1 ・ Gene ID: 208 AKT2 ・ Gene ID: 10000 AKT3 ・ UniProt: P31749 AKT1 ・ UniProt: P31751 AKT2 ・ UniProt: Q9Y243 AKT3

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REFERENCEE(抜粋)

Application Reference	Application/Reactivity
Pan CH et al. Aging (Albany NY) 2019; 11 (19) : 8085-8102 Oxidative stress-induced cellular senescence desensitizes cell growth and migration of vascular smooth muscle cells through down-regulation of platelet-derived growth factor receptor-beta.	Application : WB Reactivity : Rat
Cheng YM et al. Oncotarget 2018; 9 (41) : 26342-26352 PSMD4 is a novel therapeutic target in chemoresistant colorectal cancer activated by cytoplasmic localization of Nrf2.	Application : WB Reactivity : Human
Zhao J et al. J Steroid Biochem Mol Biol 2018; (Epub) Orchiectomy and * differentially regulate synaptic plasticity and spatial memory in a manner that is mediated by SRC-1 in the hippocampus of male mice.	Application : WB Reactivity : Mouse
Lin PL et al. Free Radic Biol Med 2016; 95 : 121-132 Cytoplasmic localization of Nrf2 promotes colorectal cancer with more aggressive tumors via upregulation of PSMD4.	Application : WB, IHC-P Reactivity : Monkey
Chun-Hao Tsai et al. Toxicology Research 2016; 5 : 151-159 Identification of Id1 as a downstream effector for arsenic-promoted angiogenesis via PI3K/Akt, NF-κB and NOS signaling.	Application : WB Reactivity : Human, Mouse

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