抗RASSF1A [3F3]抗体 | Anti-RASSF1A [3F3] antibody

• 特長
• 価格
• DATA IMAGES
• 製品情報
• APPLICATION
• PROPERTIES
• TARGET

特長

• 高品質の抗体です。
• 幅広い研究分野に関連する抗体を取り揃えています。
• 使用されたアプリケーションや動物種などの情報が充実しています。
• 多数の論文で使用実績がある信頼性の高い抗体です。

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価格

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DATA IMAGES

	Cell lysates of HEk293(1), A549(2) and HeLa(3) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human RASSF1A (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection
	A431 cells were labeled with anti-RASSF1A (clone 3F3) mAb, and detection was using a biotinylated secondary antibody and Texas-red conj streptavidin.

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製品情報

Host	Mouse
Clonality	Monoclonal
Clone Name	3F3
Isotype	IgG1
Application	WB, ICC/IF, ELISA
Reactivity	Human

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APPLICATION

Application Note

We recommend the following starting dilutions:
Western Blot: Use at 1:500 ~ 1:2,000.

Optimal working concentrations should be determined experimentally by the end user.

Calculated MW	39 kDa. ( Note )

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PROPERTIES

Form	Liquid
Buffer	Supplied in Phosphate-Buffered Saline (pH 7.4) with 0.1% Sodium Azide.
Storage	Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration	1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species	Human
Immunogen	Recombinant human RASSF1A (1-340aa) purified from E. coli
Purification	By protein-G affinity chromatography
Conjugation	Unconjugated
Note	For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

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TARGET

Synonyms	Ras Association Domain Family Member 1 , 123F2 , Nore2A , Rassf1A , Rda32 , Reh3P21 , Rassf1
Cellular Localization	Localizes to cytoplasmic microtubules during interphase, to bipolar centrosomes associated with microtubules during prophase, to spindle fibers and spindle poles at metaphase and anaphase, to the midzone during early telophase, and to the midbody in late
Background	The tumor suppressor gene RASSF1A (3p21.3), human Ras association domain family 1A, is expressed in heart, brain, placenta, lung, liver, skeletal muscle, kidney, pancreas, spleen, thymus, prostate, testis, small intestine, colon, and peripheral blood leukocytes. RASSF1A expression is inhibited by hypermethylation of the CpG island including the promoter and early transcribed regions of the human RASSF1A gene. Hypermethylated RASSF1A promoter is observed in primary tumors. Furthermore, RASSF1A is related with cell cycle regulation, apoptosis and microtubule stability.
Database	・ Gene ID: 11186 ・ UniProt: Q9NS23

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