抗ATG5抗体 | Anti-ATG5 antibody

• 特長
• 価格
• DATA IMAGES
• 製品情報
• APPLICATION
• PROPERTIES
• TARGET
• REFERENCEE(抜粋)

特長

• 高品質の抗体です。
• 幅広い研究分野に関連する抗体を取り揃えています。
• 使用されたアプリケーションや動物種などの情報が充実しています。
• 多数の論文で使用実績がある信頼性の高い抗体です。

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価格

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DATA IMAGES

	ICC/IF analysis of HeLa cells using GTX48583 ATG5 antibody. Green : primary antibodyRed : TubulinBlue : DAPI Dilution : 1:200Fixation : 10% formalinPermibilization : 0.5% Triton-X100
	WB analysis of HeLa cell lysate using GTX48583 ATG5 antibody.
	IHC-P analysis of mouse intestine tissue using GTX48583 ATG5 antibody.
	IHC-P analysis of human liver tissue using GTX48583 ATG5 antibody. Dilution : 2.5 μg/ml
	WB analysis of HeLa, A431, and MEF cell lysate using GTX48583 ATG5 antibody. Dilution : 2 μg/ml

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製品情報

Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Application	WB, ICC/IF, IHC-P, FACS, IP, PLA
Reactivity	Human, Mouse, Rat, Zebrafish, Bovine, Pig, Xenopus, Primate

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APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Dilution
WB	1:500
ICC/IF	1:250
IHC-P	1:400
FACS	Assay dependent
IP	1:10 - 1:500
PLA	Assay dependent

Not tested in other applications.

Calculated MW	32 kDa. ( Note )
Specificity/Sensitivity	This is selective for the full-length and calpain cleaved isoform proteins. The short isoform is missing amino acids 1-79. The calpain cleaved form of ATG5 is missing amino acids 195-275.

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PROPERTIES

Form	Liquid
Buffer	PBS, 0.02% Sodium Azide
Storage	Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration	Batch dependent (Please refer to the vial label for the specific concentration.)
Antigen Species	Human
Immunogen	A synthetic peptide made to an N-terminal region of the human ATG5 protein (within residues 1-50) [Swiss-Prot Q9H1Y0]
Purification	Purified by antigen-affinity chromatography
Conjugation	Unconjugated
Note	For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

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TARGET

Synonyms	autophagy related 5 , APG5 , APG5-LIKE , APG5L , ASP , SCAR25 , hAPG5
Cellular Localization	Cytoplasm
Database	・ Gene ID: 9474 ATG5 ・ UniProt: Q9H1Y0 ATG5

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REFERENCEE(抜粋)

Application Reference	Application/Reactivity
Lenain C et al. Carcinogenesis 2015; 36 (11) : 1263-74 Autophagy-mediated degradation of nuclear envelope proteins during oncogene-induced senescence.

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