抗Gli2, Internal抗体 | Anti-Gli2, Internal antibody

• 特長
• 価格
• DATA IMAGES
• 製品情報
• APPLICATION
• PROPERTIES
• TARGET

特長

• 高品質の抗体です。
• 幅広い研究分野に関連する抗体を取り揃えています。
• 使用されたアプリケーションや動物種などの情報が充実しています。
• 多数の論文で使用実績がある信頼性の高い抗体です。

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価格

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DATA IMAGES

	IHC-P analysis of human intestine tissue using GTX46056 Gli2 antibody at 4.0-8.0μg/ml.
	WB analysis of Jurkat cells using GTX46056 Gli2 antibody at 2.5μg/ml. Lane A : primary antibody Lane B : primary antibody + blocking peptide
	WB analysis of HepG2 cells using GTX46056 Gli2 antibody at 1.0μg/ml.
	IHC-P analysis of human heart tissue using GTX46056 Gli2 antibody at 4.0-8.0μg/ml.
	IHC-Fr analysis of mouse gut tissue using GTX46056 Gli2 antibody at 1:50. Green : GL2 Red : Neural crest cell. Fixed tissue was treated with 0.1% Triton X-100 for 4-6 hours at room temperature or overnight at 4ºC, washed in PBS, incubated with primary antibodies for 4-6 hours at room temperature or overnight at 4ºC, washed in PBS, and then stained with secondary antibody (Goat Anti-Rabbit Cy3) for 4-6 hours at room temperature or overnight at 4ºC.

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製品情報

Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Application	WB, IHC-P, IHC-Fr
Reactivity	Human, Mouse

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APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Dilution
WB	0.2-2.5 ug/ml
IHC-P	2-10 ug/ml
IHC-Fr	Assay dependent

Not tested in other applications.

Calculated MW	168 kDa. ( Note )
Positive Control	HepG2 , Jurkat

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PROPERTIES

Form	Liquid
Buffer	1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Storage	Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration	0.5-1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species	Human
Immunogen	A synthetic peptide corresponding to an Internal region of Human Gli2
Purification	Protein A purified
Conjugation	Unconjugated
Note	For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

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TARGET

Synonyms	GLI family zinc finger 2 , CJS , HPE9 , PHS2 , THP1 , THP2
Cellular Localization	Nucleus,Cytoplasm
Background	This gene encodes a protein which belongs to the C2H2-type zinc finger protein subclass of the Gli family. Members of this subclass are characterized as transcription factors which bind DNA through zinc finger motifs. These motifs contain conserved H-C links. Gli family zinc finger proteins are mediators of Sonic hedgehog (Shh) signaling and they are implicated as potent oncogenes in the embryonal carcinoma cell. The protein encoded by this gene localizes to the cytoplasm and activates patched Drosophila homolog (PTCH) gene expression. It is also thought to play a role during embryogenesis. The encoded protein is associated with several phenotypes- Greig cephalopolysyndactyly syndrome, Pallister-Hall syndrome, preaxial polydactyly type IV, postaxial polydactyly types A1 and B. [provided by RefSeq, Jul 2008]
Database	・ Gene ID: 2736 GLI2 ・ UniProt: P10070 GLI2

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