抗CD3 [CD3-12]抗体 | Anti-CD3 [CD3-12] antibody

• 特長
• 価格
• DATA IMAGES
• 製品情報
• APPLICATION
• PROPERTIES
• TARGET
• REFERENCEE(抜粋)

特長

• 高品質の抗体です。
• 幅広い研究分野に関連する抗体を取り揃えています。
• 使用されたアプリケーションや動物種などの情報が充実しています。
• 多数の論文で使用実績がある信頼性の高い抗体です。

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価格

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DATA IMAGES

	WB analysis of Jurkat cell lysate using GTX42110 CD3 antibody [CD3-12].
	IHC-P analysis of human tonsil tissue using GTX42110 CD3 antibody. Heat mediated antigen retrieval using EDTA buffer pH9.0.

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製品情報

Host	Rat
Clonality	Monoclonal
Clone Name	CD3-12
Isotype	IgG1
Application	WB, ICC/IF, IHC-P, IHC-Fr, FACS
Reactivity	Human, Mouse, Rabbit, Bovine, Cat, Dog, Chicken, Pig, Alpaca, Amazon Parrot, Bullfrog, Cynomolgus monkey, Duck, Great horned owl (Bubo virginianus), Harbour Porpoise, Horse, Koala, Raccoon, Rhesus Monkey, Sea Lion, Spotted Hyena, Xenopus

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APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Dilution
WB	Assay dependent
ICC/IF	Assay dependent
IHC-P	1/100
IHC-Fr	1/100
FACS	1/50-1/100
Note : IHC-P This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. Tris/EDTA buffer pH 9.0 is recommended for this purpose. FACS Membrane permeabilization is required for this application.

Not tested in other applications.

Specificity/Sensitivity	This antibody raised against a peptide representing an invariant cytoplasmic  sequence  within  the  CD3ε  chain  recognizes  human  CD3ε.

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PROPERTIES

Form	Liquid
Buffer	PBS, 0.09% Sodium Azide
Storage	Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration	1.0 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species	Human
Immunogen	Synthetic peptide sequence derived from cytoplasmic epitope of CD3 (Glu-Arg-Pro-Pro-Pro-Val-Pro-Asn-Pro-Asp-Tyr-Glu-Pro-Cys) (ERPPPVPNPDYEPC )
Purification	Purified IgG From tissue culture supernatant
Conjugation	Unconjugated
Note	For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

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TARGET

Synonyms

T3E Antibody , TCRE Antibody , CD3e molecule, epsilon (CD3-TCR complex) Antibody , CD3E Antibody

Background

The protein encoded by this gene is the CD3-epsilon polypeptide, which together with CD3-gamma, -delta and -zeta, and the T-cell receptor alpha/beta and gamma/delta heterodimers, forms the T-cell receptor-CD3 complex. This complex plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways. The genes encoding the epsilon, gamma and delta polypeptides are located in the same cluster on chromosome 11. The epsilon polypeptide plays an essential role in T-cell development. Defects in this gene cause immunodeficiency. This gene has also been linked to a susceptibility to type I diabetes in women. [provided by RefSeq, Jul 2008]

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REFERENCEE(抜粋)

Application Reference	Application/Reactivity
Kirby L et al. Nat Commun 2019; 10 (1) : 3887 Oligodendrocyte precursor cells present antigen and are cytotoxic targets in inflammatory demyelination.	Application : IHC-Fr Reactivity : Mouse
Maeda T et al. Int J Cancer 2018; (Epub) Immune-mediated antitumor effect of a transplanted lymph node.	Application : IHC-P Reactivity : Mouse
Maeda T et al. Lymphat Res Biol 2017; (Epub) Lymphatic Reconnection and Restoration of Lymphatic Flow by Nonvascularized Lymph Node Transplantation: Real-Time Fluorescence Imaging Using * Green and Fluorescein Isothiocyanate-Dextran.	Application : IHC-P Reactivity : Mouse
Ni G et al. BMC Immunology 2017; (Epub) Blocking IL-10 signalling at the time of immunization does not increase unwanted side effects in mice.	Application : FACS Reactivity : Mouse

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