抗Nestin [10C2]抗体 | Anti-Nestin [10C2] antibody

• 特長
• 価格
• DATA IMAGES
• 製品情報
• APPLICATION
• PROPERTIES
• TARGET
• REFERENCEE(抜粋)

特長

• 高品質の抗体です。
• 幅広い研究分野に関連する抗体を取り揃えています。
• 使用されたアプリケーションや動物種などの情報が充実しています。
• 多数の論文で使用実績がある信頼性の高い抗体です。

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価格

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DATA IMAGES

	ICC/IF analysis of F9 cells using GTX30671 Nestin antibody [10C2]. Green : primary antibody(left) and isotype control(right)Blue : DAPI Dilution : 1:50Permibilization : 0.1% Triton X-100
	FACS (Intracellular staining) analysis of 1 x 10⁶ CHO (A) and HEK293 (B) cells using GTX30671 Nestin antibody [10C2]. Blue : Primary antibody Orange : isotype controlDilution : 1 μg/10⁶ cells
	IHC-P analysis of human kidney tissue using GTX30671 Nestin antibody [10C2]. Dilution : 1:500
	WB analysis of (1) rat brain tissue, and (2) human CNS progenitor cell lysate using GTX30671 Nestin antibody [10C2]. The antibody did not react with Rat, whereas, in lysate from human samples, it generated a doublet band representing the phosphorylated and the non-phosphorylated forms of Nestin protein.Loading : 5μgDilution : 1:1000

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製品情報

Host	Mouse
Clonality	Monoclonal
Clone Name	10C2
Isotype	IgG1
Application	WB, ICC/IF, IHC-P, IHC-Fr, FACS, MS
Reactivity	Human, Hamster

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APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Dilution
WB	1:1000 - 1:5000
ICC/IF	1:50 - 1:200
IHC-P	1:100 - 1:200
IHC-Fr	1:100 - 1:200
FACS	1 μg / 10⁶ cells
MS	Assay dependent

Not tested in other applications.

Calculated MW	177 kDa. ( Note )
Specificity/Sensitivity	This product does not react with Mouse,Rat,Rabbit.

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PROPERTIES

Form	Liquid
Buffer	PBS, 0.1% Sodium Azide
Storage	Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration	2.4 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species	Human
Immunogen	A fragment of the human Nestin protein corresponding to residues 1464-1614. [UniProt P48681]
Purification	Protein G purified
Conjugation	Unconjugated
Note	For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

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TARGET

Synonyms	nestin , Nbla00170
Background	Nestin is an intermediate filament protein that was first identified with a monoclonal antibody by Hockfield and McKay (1985) [PubMed 4078630]. It is expressed predominantly in stem cells of the central nervous system in the neural tube. Upon terminal neural differentiation, nestin is downregulated and replaced by neurofilaments.[supplied by OMIM]
Database	・ Gene ID: 10763 NES ・ UniProt: P48681 NES

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REFERENCEE(抜粋)

Application Reference	Application/Reactivity
Lu P et al. J Clin Invest 2017; 127 (9) : 3287-3299 Prolonged human neural stem cell maturation supports recovery in injured rodent CNS.	Application : IHC-Fr Reactivity : Rat
Garcia-Castro IL et al. PLoS One 2015; 10 (12) : e0146082 Markers of Pluripotency in Human Amniotic Epithelial Cells and Their Differentiation to Progenitor of Cortical Neurons.

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