抗HIF1 alpha抗体 | Anti-HIF1 alpha antibody

• 特長
• 価格
• DATA IMAGES
• 製品情報
• APPLICATION
• PROPERTIES
• TARGET
• REFERENCEE(抜粋)

特長

• 高品質の抗体です。
• 幅広い研究分野に関連する抗体を取り揃えています。
• 使用されたアプリケーションや動物種などの情報が充実しています。
• 多数の論文で使用実績がある信頼性の高い抗体です。

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価格

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DATA IMAGES

	WB analysis of hypoxia-treated sample using GTX30647 HIF1 alpha antibody. Lane 1 : CoCl2 treated Cos-7 nuclear extractLane 2 : Untreated Cos-7 nuclear extractLoading : 50μg
	IHC-P analysis of human kidney cancer tissue using GTX30647 HIF1 alpha antibody.
	WB analysis of (A)PC12 cells, 20% oxygen and (B)PC12 cells, 1% oxygen using GTX30647 HIF1 alpha antibody.
	IHC-P analysis of human placenta tissue using GTX30647 HIF1 alpha antibody.
	ICC/IF analysis of RCC4 cells using GTX30647 HIF1 alpha antibody. Green : primary antibodyBlue : Hoechst 33258

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製品情報

Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Application	WB, ICC/IF, IHC-P, IHC-Fr, FACS, IP, ChIP assay
Reactivity	Human, Mouse, Rat, Rabbit, Goat, Hamster, Monkey, Primate

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APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Dilution
WB	1:500 - 1:2000
ICC/IF	1:100
IHC-P	5 - 10 μg/ml
IHC-Fr	5 - 10 μg/ml
FACS	1:10 - 1:1000
IP	1:10 - 1:500
ChIP assay	1:10 - 1:500

Not tested in other applications.

Calculated MW	94 kDa. ( Note )

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PROPERTIES

Form	Liquid
Buffer	PBS, 0.05% Sodium Azide
Storage	Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC. DO NOT FREEZE.
Concentration	1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species	Mouse
Immunogen	A fusion protein including amino acids 530 - 825 of the mouse HIF-1 alpha protein [UniProt# Q61221].
Purification	Purified by antigen-affinity chromatography
Conjugation	Unconjugated
Note	For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

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TARGET

Synonyms	hypoxia inducible factor 1, alpha subunit , AA959795 , HIF-1-alpha , HIF1-alpha , HIF1alpha , MOP1 , bHLHe78
Cellular Localization	Cytoplasm,Nucleus,Nucleus speckle
Background	Functions as a master transcriptional regulator of the adaptive response to hypoxia. Under hypoxic conditions activates the transcription of over 40 genes, including, erythropoietin, glucose transporters, glycolytic enzymes, vascular endothelial growth factor, and other genes whose protein products increase oxygen delivery or facilitate metabolic adaptation to hypoxia. Plays an essential role in embryonic vascularization, tumor angiogenesis and pathophysiology of ischemic disease. Binds to core DNA sequence 5'-[AG]CGTG-3' within the hypoxia response element (HRE) of target gene promoters. Activation requires recruitment of transcriptional coactivators such as CREBPB and EP300. Activity is enhanced by interaction with both, NCOA1 or NCOA2. Interaction with redox regulatory protein APEX seems to activate CTAD and potentiates activation by NCOA1 and CREBBP.
Database	・ Gene ID: 15251 Hif1a ・ UniProt: Q61221 Hif1a

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REFERENCEE(抜粋)

Application Reference	Application/Reactivity
Jing L et al. Exp Ther Med 2015; 9 (6) : 2141-2146 Akt/hypoxia-inducible factor-1α signaling deficiency compromises skin wound healing in a type 1 diabetes mouse model.	Application : WB Reactivity : Mouse
Weir L et al. Biochim Biophys Acta 2011; 1813 (1) : 60-72 Hypoxia-mediated control of HIF/ARNT machinery in epidermal keratinocytes.	Application : WB Reactivity : Human

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