抗HIF1 alpha [H1alpha67]抗体 | Anti-HIF1 alpha [H1alpha67] antibody

• 特長
• 価格
• DATA IMAGES
• 製品情報
• APPLICATION
• PROPERTIES
• TARGET
• REFERENCEE(抜粋)

特長

• 高品質の抗体です。
• 幅広い研究分野に関連する抗体を取り揃えています。
• 使用されたアプリケーションや動物種などの情報が充実しています。
• 多数の論文で使用実績がある信頼性の高い抗体です。

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価格

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DATA IMAGES

	WB analysis of cobalt chloride treated COS-7 nuclear extracts using GTX30105 HIF1 alpha antibody [H1alpha67]. Loading : 50μg
	IHC-P analysis of human kidney tissue using GTX30105 HIF1 alpha antibody [H1alpha67].
	IHC-P analysis of human glioblastoma multiforme tissue using GTX30105 HIF1 alpha antibody [H1alpha67].
	ICC/IF analysis of Deferoxamine-treated HeLa cells using GTX30105 HIF1 alpha antibody [H1alpha67]. Red : primary antibodyBlue : DAPI Dilution : 1 μg/ml

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製品情報

Host	Mouse
Clonality	Monoclonal
Clone Name	H1alpha67
Isotype	IgG2b
Application	WB, ICC/IF, IHC-P, IHC-Fr, FACS, IP, ELISA, ChIP assay, Activation, Gel supershift assays, IHC
Reactivity	Human, Mouse, Rat, Yeast, Rabbit, Sheep, Bovine, Pig, Monkey, Bat, Ferret, Xenopus, Primate

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APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Dilution
WB	1:500
ICC/IF	1:50
IHC-P	1:20 - 1:50
IHC-Fr	1:20 - 1:50
FACS	1:10 - 1:1000
IP	1:10 - 1:500
ELISA	1:100 - 1:2000
ChIP assay	1 - 5 μg
Activation	Assay dependent
Gel supershift assays	1:1 - 1:100
IHC	1:20 - 1:50

Not tested in other applications.

Calculated MW	93 kDa. ( Note )

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PROPERTIES

Form	Liquid
Buffer	PBS, 1% BSA, 0.05% Sodium Azide
Storage	Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration	1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species	Human
Immunogen	A fusion protein containing amino acids 432 - 528 of human HIF-1 alpha [UniProt# Q16665].
Purification	Protein G purified
Conjugation	Unconjugated
Note	For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

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TARGET

Synonyms	hypoxia inducible factor 1 subunit alpha , HIF-1-alpha , HIF-1A , HIF-1alpha , HIF1 , HIF1-ALPHA , MOP1 , PASD8 , bHLHe78
Cellular Localization	Cytoplasm,Nucleus,Nucleus speckle
Background	Hypoxia-inducible factor-1 (HIF1) is a transcription factor found in mammalian cells cultured under reduced oxygen tension that plays an essential role in cellular and systemic homeostatic responses to hypoxia. HIF1 is a heterodimer composed of an alpha subunit and a beta subunit. The beta subunit has been identified as the aryl hydrocarbon receptor nuclear translocator (ARNT). This gene encodes the alpha subunit of HIF-1. Overexpression of a natural antisense transcript (aHIF) of this gene has been shown to be associated with nonpapillary renal carcinomas. Two alternative transcripts encoding different isoforms have been identified. [provided by RefSeq]
Database	・ Gene ID: 3091 HIF1A ・ UniProt: Q16665 HIF1A

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REFERENCEE(抜粋)

Application Reference	Application/Reactivity
Garc?a G?mez-Heras S et al. PeerJ 2019; 7 : e7160 Main histological parameters to be evaluated in an experimental model of myocardial infarct treated by stem cells on pigs.	Application : IHC-P Reactivity : Pig
Garcia-Vasquez C et al. Exp Biol Med (Maywood) 2018; 243 (10) : 803-808 Fibrin patch influences the expression of hypoxia-inducible factor-1α and nuclear factor-κBp65 factors on ischemic intestinal anastomosis.	Application : IHC-P Reactivity : Pig
Lin CL et al. Neuroreport 2017; 28 (15) : 956-962 Hypothermic neuroprotections in the brain of an echolocation bat, Hipposideros terasensis.	Application : IHC Reactivity : Bat
Roan JN et al. J Surg Res 2017; 208 : 93-103 Exendin-4, a glucagon-like peptide-1 analogue, accelerates diabetic wound healing	Application : WB Reactivity : Rat
Mung KL et al. Stem Cell Res Ther 2016; 7 (1) : 146 Rapid and efficient generation of neural progenitors from adult bone marrow stromal cells by hypoxic preconditioning.	Application : WB Reactivity : Rat

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