抗AKT (phospho Thr308)抗体 | Anti-AKT (phospho Thr308) antibody

• 特長
• 価格
• DATA IMAGES
• 製品情報
• APPLICATION
• PROPERTIES
• TARGET
• REFERENCEE(抜粋)

特長

• 高品質の抗体です。
• 幅広い研究分野に関連する抗体を取り揃えています。
• 使用されたアプリケーションや動物種などの情報が充実しています。
• 多数の論文で使用実績がある信頼性の高い抗体です。

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価格

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DATA IMAGES

Western Blot of Rabbit anti-Akt phospho T308 antibody (GTX28933). Lane 1: GST tagged AKT1 un-active recombinant protein. Lane 2: GST tagged AKT1 active recombinant protein. Load: 50 ng per lane. Primary antibody: Akt phospho T308 antibody at 1:1,000 for overnight at 4ºC. Secondary antibody: DyLight™ 649 rabbit secondary antibody at 1:20,000 for 30 min at RT. Block for 30 min at RT. Other band(s): none.

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製品情報

Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Application	WB, FACS, ELISA
Reactivity	Human, Mouse, Rat

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APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Dilution
WB	Assay dependent
FACS	Assay dependent
ELISA	1:15000

Not tested in other applications.

Calculated MW	56 kDa. ( Note )

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PROPERTIES

Form	Liquid
Buffer	0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 and 0.01% (w/v) Sodium Azide
Storage	Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration	0.44 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species	Human
Immunogen	Synthetic peptide: DGATMKTpFCG conjugated to KLH, corresponding to amino acids 302-311 of AKT.
Purification	Purified by antigen-affinity chromatography. This product was prepared from monospecific antiserum by immunoaffinity chromatography using phospho peptide coupled to agarose beads followed by solid phase adsorption(s) against non-phospho peptide and non-specific peptide to remove any unwanted reactiv
Conjugation	Unconjugated
Note	For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

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TARGET

Synonyms	AKT serine/threonine kinase 1 , AKT , CWS6 , PKB , PKB-ALPHA , PRKBA , RAC , RAC-ALPHA
Cellular Localization	Cytoplasm,Nucleus,Cell membrane
Background	Akt (Protein Kinase B, PKB) is a key regulator of many signal transduction pathways. Its tight control over cell proliferation and cell viability are manifold; overexpression or inappropriate activation of Akt has been seen in many types of cancer. Akt mediates many of the downstream events of PI 3-kinase (a lipid kinase activated by growth factors, cytokines and insulin). PI 3-kinase recruits Akt to the membrane, where it is activated by PDK1 phosphorylation. Once phosphorylated, Akt dissociates from the membrane and phosphorylates targets in the cytoplasm and the cell nucleus. Akt has two main roles: (i) inhibition of apoptosis; (ii) promotion of proliferation.
Database	・ Gene ID: 207 AKT1 ・ UniProt: P31749 AKT1

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REFERENCEE(抜粋)

Application Reference	Application/Reactivity
Liu J et al. Mol Med Rep 2019; 20 (1) : 33-40 miR?338?3p inhibits A549 lung cancer cell proliferation and invasion by targeting AKT and β?catenin signaling pathways.	Application : WB Reactivity : Human
Piva MBR et al. Cancers (Basel) 2017; 9 (9) integrin activation contributes to lower * sensitivity in mv3 melanoma cells by inducing the wnt signalling pathway.	Application : FACS Reactivity : Human
Chen CY et al. Oncotarget 2015; 6 (29) : 26946-59 Targeting annexin A2 reduces tumorigenesis and therapeutic resistance of nasopharyngeal carcinoma.	Application : WB Reactivity : Human
Matsuda N et al. Cardiovasc Res 2007; 76 (1) : 132-40 Silencing of caspase-8 and caspase-3 by RNA interference prevents vascular endothelial cell injury in mice with endotoxic shock.	Application : WB Reactivity : Mouse

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