抗NOTCH1抗体 | Anti-NOTCH1 antibody

• 特長
• 価格
• DATA IMAGES
• 製品情報
• APPLICATION
• PROPERTIES
• TARGET
• REFERENCEE(抜粋)

特長

• 高品質の抗体です。
• 幅広い研究分野に関連する抗体を取り揃えています。
• 使用されたアプリケーションや動物種などの情報が充実しています。
• 多数の論文で使用実績がある信頼性の高い抗体です。

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価格

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DATA IMAGES

	Rabbit anti-Human NOTCH 1 (Cleaved N Terminal) was used at a 1:500 dilution to detect mouse Notch 1 by Western blot in 293 cells transiently transfected with myc-tagged mouse Notch. Bound antibody was detected with a 1:10,000 dilution of HRP conjugated Goat anti Rabbit IgG, and color development. Lane M: markers. Lane 1: No transfection. Lane 2: N1 (mouse deleted extracellular domain)-myc. Lane 3: N1 (mouse intracellular domain)-myc. Lane 4: N2 (mouse deleted extracellular domain)-myc. Lane 5: N2 (mouse intracellular domain)-myc. Lane 6: N3 (mouse deleted extracellular domain)-myc. Lane 7: N3 (mouse intracellular domain)-myc. Lane 8: N4 (mouse deleted extracellular domain)-myc. Lane 9: N4 (mouse intracellular domain)-myc. Lane 10: N1 (mouse deleted extracellular domain)(V to G)-myc.
	Western Blot of Rabbit anti-Notch1 antibody (GTX28925). Lane 1: MCF-7 control lysate. Lane 2: MCF-7 +1 nM 17β-estradiol. Lane 3: MCF-7 + 10 μM gamma secretase inhibitor. Load: 35 μg per lane. Primary antibody: Notch1 antibody at 1:500 for overnight at 4ºC. Rabbit secondary antibody at 1:10,000 for 45 min at RT.Predicted/Observed size: 80 kDa for Notch1.
	IHC-P analysis of exocrine glands of human pancreas using Rabbit anti-Notch1 antibody (GTX28925) at a dilution of 1:200. Staining: moderate to strong membranous staining and faint to moderate cytoplasmic staining. Islets showed faint staining.

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製品情報

Host	Rabbit
Clonality	Polyclonal
Application	WB, ICC/IF, IHC-P, ELISA, Neutralizing/Blocking
Reactivity	Human, Mouse, Rat

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APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Dilution
WB	1:500 - 1:2000
ICC/IF	Assay dependent
IHC-P	1:200
ELISA	1:20000 - 1:60000
Neutralizing/Blocking	Assay dependent

Not tested in other applications.

Calculated MW	273 kDa. ( Note )
Specificity/Sensitivity	This antibody reacts with mouse Notch constructs present in lysates of HEK 293 cells.  Only the cleaved intracellular (activated) form (NICD) is detected.  No reactivity is detected against mouse N2, N3 or N4.  The immunogen epitope is only exposed after gamma secretase cleavage and is not accessible in the uncleaved form.

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PROPERTIES

Form	Liquid
Buffer	0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 and 0.1% (w/v) Sodium Azide
Storage	Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration	78 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species	Human
Immunogen	Peptide: VLLSRKRRRQHGQC (aa1755-1767).This is the N-terminal sequence of the cleaved N intracellular domain (NICD).
Purification	Antiserum
Conjugation	Unconjugated
Note	For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

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TARGET

Synonyms	notch receptor 1 , AOS5 , AOVD1 , TAN1 , hN1
Cellular Localization	Cell membrane
Background	Notch 1 is synthesized in the endoplasmic reticulum as an inactive form, which is proteolytically cleaved by a furin-like convertase (S1 cleavage) in the trans-golgi network before it reaches the plasma membrane to yield an active, ligand-accessible form. Cleavage results in a C-terminal fragment N(TM) and a N-terminal fragment N(EC). Following ligand binding, it is cleaved (S2 cleavage) by TNF-alpha converting enzyme (TACE) to yield a membrane-associated intermediate fragment called Notch extracellular truncation (NEXT). This fragment is then cleaved by presenilin-dependent gamma-secretase (S3 cleavage) to release the intracellular domain (NICD) from the membrane.
Database	・ Gene ID: 4851 NOTCH1 ・ UniProt: P46531 NOTCH1

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REFERENCEE(抜粋)

Application Reference	Application/Reactivity
Nwabo Kamdje AH et al. Blood Cancer J 2012; 2 (5) : e73 Role of stromal cell-mediated Notch signaling in CLL resistance to chemotherapy.	Application : Neutralizing/Blocking
Chu PW et al. Fertil Steril 2011; 96 (5) : 1225-9 Notch 1 signaling pathway effect on implantation competency.	Application : WB Reactivity : Mouse
Cheng X et al. Development 2008; 135 (20) : 3447-58 Numb mediates the interaction between Wnt and Notch to modulate primitive erythropoietic specification from the hemangioblast.	Application : ICC/IF Reactivity : Mouse

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