抗AKT抗体 | Anti-AKT antibody

• 特長
• 価格
• DATA IMAGES
• 製品情報
• APPLICATION
• PROPERTIES
• TARGET
• REFERENCEE(抜粋)

特長

• 高品質の抗体です。
• 幅広い研究分野に関連する抗体を取り揃えています。
• 使用されたアプリケーションや動物種などの情報が充実しています。
• 多数の論文で使用実績がある信頼性の高い抗体です。

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価格

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DATA IMAGES

	Western Blot of simultaneous detection of unphosphorylated and phosphorylated Rabbit Anti-AKT antibody (GTX28805). Lane 1: unstimulated NIH/3T3 lysates contain inactive unphosphorylated Akt1, green band. Lane 2: PDGF stimulated NIH/3T3 lysate contains both inactive (green band) and activated phosphorylated Akt1 (red band). Load: 35 μg per lane. Primary antibody: rabbit anti-Akt (pan) and mouse anti-Akt pS473 specific antibodies at 1:1000 for overnight at 4ºC. Secondary antibody: Flourescent 549 conjugated anti-rabbit IgG (green) and flourescent 649 conjugated anti-mouse IgG (red) secondary antibodies at 1:10,000 for 45 min at RT.
	Immunohistochemical analysis of formalin-fixed paraffin-embedded tissue, A: Normal colon tissue, B: Colon tumor tissue, using AKT(GTX28805) antibody at 1:1,000 dilution for 1 h at RT. Peroxidase rabbit secondary antibody at 1:10,000 for 45 min at RT. Localization: AKT is nuclear. Staining: AKT as precipitated red signal with hematoxylin purple nuclear counterstain.
	ICC/IF of the Akt antibody is used at 1: 80 for 1hr at RT on cultured neonatal rat cardiomyocytes (Green). The red is Texas-red phalloidin that labels actin filaments.
	Western blot using Akt antibody at 1:500 for overnight at 4ºC on 20 ug NIH/3T3 whole cell lysate.

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製品情報

Host	Rabbit
Clonality	Polyclonal
Application	WB, ICC/IF, IHC-P, FACS, ELISA
Reactivity	Human, Mouse, Rat, Chicken

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APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Dilution
WB	1:500 - 1:2000
ICC/IF	1:100 - 1:1000
IHC-P	1:500 - 1:2000
FACS	Assay dependent
ELISA	1:2000 - 1:10000

Not tested in other applications.

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PROPERTIES

Form	Liquid
Buffer	0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 and 0.01% (w/v) Sodium Azide
Storage	Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration	75 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species	Human
Immunogen	AKT Antibody was produced from whole rabbit serum prepared by repeated immunizations with a synthetic peptide C-R-P-H-F-P-Q-F-S-Y-S-A-S-G-T-A corresponding to the C-terminus (460-480) of human, rat and mouse and chicken AKT proteins conjugated to KLH usin
Purification	Antiserum
Conjugation	Unconjugated
Note	For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

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TARGET

Background

Akt (Protein Kinase B, PKB) is a key regulator of many signal transduction pathways (reviewed in ref 1). Its tight control over cell proliferation and cell viability are manifold; overexpression or inappropriate activation of Akt has been seen in many types of cancer. Akt mediates many of the downstream events of PI 3-kinase (a lipid kinase activated by growth factors, cytokines and insulin). PI 3-kinase recruits Akt to the membrane, where it is activated by PDK1 phosphorylation (reviewed in 2). Once phosphorylated, Akt dissociates from the membrane and phosphorylates targets in the cytoplasm and the cell nucleus. Akt has two main roles: (i) inhibition of apoptosis; (ii) promotion of proliferation.

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REFERENCEE(抜粋)

Application Reference	Application/Reactivity
Zhao J et al. J Steroid Biochem Mol Biol 2018; (Epub) Orchiectomy and * differentially regulate synaptic plasticity and spatial memory in a manner that is mediated by SRC-1 in the hippocampus of male mice.	Application : WB Reactivity : Mouse
Qu J et al. Clin Exp Pharmacol Physiol 2015; (Epub) PFKFB3 modulates glycolytic metabolism and alleviates endoplasmic reticulum stress in human osteoarthritis cartilage.	Application : WB Reactivity : Human

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