抗GAPDH [6C5]抗体 | Anti-GAPDH [6C5] antibody

• 特長
• 価格
• DATA IMAGES
• 製品情報
• APPLICATION
• PROPERTIES
• TARGET
• REFERENCE

特長

• 高品質の抗体です。
• 幅広い研究分野に関連する抗体を取り揃えています。
• 使用されたアプリケーションや動物種などの情報が充実しています。
• 多数の論文で使用実績がある信頼性の高い抗体です。

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価格

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DATA IMAGES

	Immunoprecipitation of GAPDH from rat heart extract using anti-GAPDH MAb 6C5(GTX28245) (A). Mixture of protein A-Sepharose with anti-GAPDH MAbs and tissue extract was incubated for 30 min at room temperature and precipitated by centrifugation. Pellet was washed with PBS, suspended in reducing electrophoresis sample buffer and heated for 5 minutes at 100 ºC. After centrifugation supernatant was loaded on gel and proteins were separated by SDS electrophoresis. Lane 1: Human GAPDH (1 μg) Lane 2: GAPDH immunoprecipitated from rat heart tissue extract Lane 3: Only GTX28245 preincubated with Protein A Sepharose Lane 4: Only Protein A Sepharose
	The WB analysis of GAPDH antibody [6C5] was published by Brügger V and colleagues in the journal Nat Commun in 2017.PMID: 28139683
	The WB analysis of GAPDH antibody [6C5] was published by Brügger V and colleagues in the journal Nat Commun in 2017.PMID: 28139683
	The WB analysis of GAPDH antibody [6C5] was published by Brügger V and colleagues in the journal Nat Commun in 2017.PMID: 28139683
	The WB analysis of GAPDH antibody [6C5] was published by Brügger V and colleagues in the journal Nat Commun in 2017.PMID: 28139683
	The WB analysis of GAPDH antibody [6C5] was published by Brügger V and colleagues in the journal Nat Commun in 2017.PMID: 28139683
	The WB analysis of GAPDH antibody [6C5] was published by Brügger V and colleagues in the journal Nat Commun in 2017.PMID: 28139683
	Heart extracts were prepared from different animal species. About 0.5 mg of homogenized wet cardiac tissue per track was loaded. Lane 1: isolated human GAPDH, 0.5 μg Lane 2: human heart tissue extract Lane 3: pig heart tissue extract Lane 4: goat heart tissue extract Lane 5: bovine heart tissue extract Lane 6: dog heart tissue extract Lane 7: mouse heart tissue extract Lane 8: rat heart tissue extract Lane 9: rabbit heart tissue extract
	Immunostaining of GAPDH in A-10 cell line (rat aortic smooth muscle cells). Cells were fixed by formalin and GAPDH was stained by: GTX28245 (green colour) F-actin microfilaments-bindingdye (red) DNA-binding dye (dark blue)

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製品情報

Host	Mouse
Clonality	Monoclonal
Clone Name	6C5
Isotype	IgG1
Application	WB, ICC/IF, IP
Reactivity	Human, Mouse, Rat, Rabbit, Cat, Dog, Pig, Fish

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APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Dilution
WB	Assay dependent
ICC/IF	Assay dependent
IP	Assay dependent

Not tested in other applications.

Calculated MW	36 kDa. ( Note )

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PROPERTIES

Form	Liquid
Buffer	PBS pH 7.4, 0.09 % sodium azide
Storage	Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4º C.
Concentration	Batch dependent (Please refer to the vial label for the specific concentration.)
Antigen Species	Rabbit
Immunogen	Rabbit muscle GAPDH
Purification	Protein A purified
Conjugation	Unconjugated
Note	For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

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TARGET

Cellular Localization	Cytoplasm, cytosol,Nucleus,Cytoplasm, cytoskeleton
Database	・ Gene ID: 100009074 GAPDH ・ UniProt: P46406 GAPDH

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REFERENCE

Application Reference	Application/Reactivity
Beauchemin M et al. Pharmacol Res 2020; 152 : 104589 Exploring mechanisms of increased cardiovascular disease risk with antipsychotic medications: Risperidone alters the cardiac proteomic signature in mice.	Application : WB Reactivity : Mouse
Roesler MK et al. Front Cell Neurosci 2019; 13 : 330 Myosin XVI Regulates Actin Cytoskeleton Dynamics in Dendritic Spines of Purkinje Cells and Affects Presynaptic Organization.	Application : WB
Tang X et al. Sci Rep 2019; 9 (1) : 15108 Tumour-Secreted Hsp90帢 on External Surface of Exosomes Mediates Tumour - Stromal Cell Communication via Autocrine and Paracrine Mechanisms.	Application : WB Reactivity : Human
Lombino FL et al. Sci Rep 2019; 9 (1) : 15940 The Microtubule Severing Protein Katanin Regulates Proliferation of Neuronal Progenitors in Embryonic and Adult Neurogenesis.	Application : WB Reactivity : Mouse
Sean C. Shadle et al. bioRxiv 2019; (Epub) DUX4-induced bidirectional HSATII satellite repeat transcripts form intranuclear double stranded RNA foci in human cell models of FSHD.	Application : WB Reactivity : Human

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