抗p27 Kip1抗体 | Anti-p27 Kip1 antibody

• 特長
• 価格
• DATA IMAGES
• 製品情報
• APPLICATION
• PROPERTIES
• TARGET
• REFERENCEE(抜粋)

特長

• 高品質の抗体です。
• 幅広い研究分野に関連する抗体を取り揃えています。
• 使用されたアプリケーションや動物種などの情報が充実しています。
• 多数の論文で使用実績がある信頼性の高い抗体です。

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価格

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DATA IMAGES

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製品情報

Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Application	WB, IHC-P, IP, IHC
Reactivity	Human, Mouse, Rat

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APPLICATION

Application Note

IHC-P: Use at an assay dependent dilution. IP: Use at an assay dependent dilution. WB: Use at an assay dependent dilution. Detects a band of approximately 27 kDa (predicted molecular weight: 22 kDa). Optimal dilutions/concentrations should be determined by the end user.

Calculated MW	22 kDa. ( Note )
Positive Control	Jurkat, HeLa, NIH/3T3, and KNRK
Specificity/Sensitivity	Reacts with p27 (also designated Kip1 p27)

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PROPERTIES

Form	Liquid
Buffer	PBS containing 0.1 % sodium azide and 0.2% gelatin
Storage	Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC. DO NOT FREEZE.
Concentration	0.2 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species	Human
Immunogen	Synthetic peptide corresponding to C terminal of Human p27.
Purification	IgG fraction
Conjugation	Unconjugated
Note	For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

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TARGET

Synonyms	Cyclin Dependent Kinase Inhibitor 1B , Cdkn4 , Kip1 , Men1B , Men4 , P27Kip1 , Cdkn1B
Cellular Localization	Nuclear and cytoplasmic in quiescent cells
Background	Cell cycle progression is regulated by cyclins and their cognate Cdks. p27 KIP 1 is a cell cycle regulatory mitotic inhibitor of cdk activity. p27 KIP 1 is a candidate tumor suppressor gene, and has been proposed to function as a possible mediator of TGF beta induced G1 arrest. p27 KIP 1 is up-regulated in response to antimitogenic stimuli. The increased protein expression of p27 results in cellular arrest by binding to cyclin/Cdk complexes such as cyclin D1/Cdk4. p27 Kip1 is regulated by phosphorylation on serine 10 (S10) and threonine 187 (T187). Phosphorylation by CDK2 on T187 results in ubiquitylation and degradation of p27 Kip 1; while phosphorylation by hKIS on S10 signals the nuclear export to the cytoplasm.
Database	・ Gene ID: 1027 ・ UniProt: P46527

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REFERENCEE(抜粋)

Application Reference	Application/Reactivity
Bellingham M et al. Mol Cell Endocrinol 2013; 376 (43102) : 156-72 Exposure to chemical cocktails before or after conception--- the effect of timing on ovarian development.
Chen YJ et al. Cancer Lett 2012; 319 (2) : 223-31 Progesterone receptor is involved in 2,3,7,8-tetrachlorodibenzo-p-dioxin-stimulated breast cancer cells proliferation.

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