抗p21 Cip1抗体 | Anti-p21 Cip1 antibody

• 特長
• 価格
• DATA IMAGES
• 製品情報
• APPLICATION
• PROPERTIES
• TARGET
• REFERENCE

特長

• 高品質の抗体です。
• 幅広い研究分野に関連する抗体を取り揃えています。
• 使用されたアプリケーションや動物種などの情報が充実しています。
• 多数の論文で使用実績がある信頼性の高い抗体です。

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価格

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DATA IMAGES

	Primary antibody (at 1:200) used to detect p21 expression by western blot. Samples 15μg each of A) A431 lysate B) HL-60 lysate C) Hela lysate
	The WB analysis of p21 Cip1 antibody was published by Lee WJ and colleagues in the journal Int J Mol Sci in 2016 . PMID: 27314341

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製品情報

Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Application	WB, IHC-Fr, IP
Reactivity	Human, Mouse, Rat

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APPLICATION

Application Note

IHC-Fr: Use at an assay dependent concentration. IP: Use at an assay dependent concentration. WB: Use at an assay dependent concentration. Can be blocked with GTX28015 p21 (C-term) peptide (human) . Optimal dilutions/concentrations should be determined by the end user.

Calculated MW	18 kDa. ( Note )
Positive Control	C32
Specificity/Sensitivity	Reacts with p21(also designated WAF1/Cip1)

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PROPERTIES

Form	Liquid
Buffer	PBS with 0.1% sodium azide
Storage	Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC. DO NOT FREEZE.
Concentration	0.2 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species	Human
Immunogen	Synthetic peptide (Human) (C terminal).
Purification	IgG fraction
Conjugation	Unconjugated
Note	For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

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TARGET

Synonyms	Cyclin Dependent Kinase Inhibitor 1A , Cap20 , Cdkn1 , Cip1 , Mda-6 , P21 , Sdi1 , Waf1 , P21Cip1 , Cdkn1A
Cellular Localization	Nuclear
Background	Cell cycle progression is regulated by cyclins and their cognate Cdks (1-3). p21 (WAF1/Cip1) inhibits the activity of each member of the cyclin/Cdk family and over expression of this protein inhibits the proliferation of mammalian cells (5). The expression of p21 is inducible by a wide range of stress stimuli and its transcription can be enhanced by p53 (6). Another member of the CDIs is p27 (also known as Kip1), which also sees up regulation in response to antimitogenic stimuli (7). The increased protein expression of p27 results in cellular arrest by binding to cyclin/Cdk complexes, like cyclin D1/Cdk4 (4,8). An additional CDI has been found to bind Cdk4 and Cdk6, p16 (INK4A), and when such complexes are formed, the progression of the cell cycle is halted (9). It has become increasingly clear that p16 is a very important tumor suppressor gene whose frequent loss occurs early in many human cancers. p16 is a major target in carcinogenesis that is rivaled in frequency only by p53 (10).
Database	・ Gene ID: 1026 ・ UniProt: P38936

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REFERENCE

Application Reference	Application/Reactivity
Lee WJ et al. Int J Mol Sci 2016; 17 (6) The Effect of MCP-1/CCR2 on the Proliferation and Senescence of Epidermal Constituent Cells in Solar Lentigo.	Application : WB Reactivity : Human
Chiu YS et al. Hepatology 2014; (Epub) IL-20 and IL-20R1 antibodies protect against liver fibrosis.	Application : WB Reactivity : Rat

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