抗Gli2抗体 | Anti-Gli2 antibody

• 特長
• 価格
• DATA IMAGES
• 製品情報
• APPLICATION
• PROPERTIES
• TARGET
• REFERENCEE(抜粋)

特長

• 高品質の抗体です。
• 幅広い研究分野に関連する抗体を取り揃えています。
• 使用されたアプリケーションや動物種などの情報が充実しています。
• 多数の論文で使用実績がある信頼性の高い抗体です。

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価格

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DATA IMAGES

	GeneTex's Affinity Purified anti-mouse Gli-2 antibody (GTX27195) was used at 10 μg/ml to evaluate staining on several mouse tissues. Moderate to strong staining was seen on many tissues, with low background staining. This image shows Gli-2 staining of mouse testis. Tissue was formalin-fixed and paraffin embedded.
	GeneTex's Affinity Purified anti-mouse Gli-2 antibody (GTX27195) was used at 10 μg/ml to evaluate staining on several mouse tissues. Moderate to strong staining was seen on many tissues with low background staining. This image shows Gli-2 staining of mouse brain. Tissue was formalin-fixed and paraffin embedded.
	Western blot using GeneTex's Affinity Purified anti-Gli-2 antibody shows detection of a predominant band at ~190 kDa corresponding to Gli-2 (arrowhead) in mouse brain whole cell lysate (lane 1). Pre-incubation of antibody with immunizing peptide completely blocks staining of this band (lane 2). ~ 25 μg of lysate was resolved on a 4-8% Tris-glycine gel by SDS-PAGE and transferred onto nitrocellulose. After blocking with 5% goat serum and 0.5% BLOTTO in PBS, the membrane was probed with the primary antibody diluted to 1:750. Incubation was at room temperature for 2 h followed by washes and reaction with a 1:10,000 dilution of IRDye® 800 conjugated Gt-a-Rabbit IgG (H&L) MX10 for 45 min at room temperature. Molecular weight markers are shown (M) using the 700 nm channel (red). IRDye® 800 fluorescence image was captured using the Odyssey® Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results.

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製品情報

Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Application	WB, IHC-P, IHC-Fr, ELISA, IHC
Reactivity	Human, Mouse

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APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Dilution
WB	1:500 - 1:2000
IHC-P	2 μg/ml-20 μg/ml
IHC-Fr	Assay dependent
ELISA	1:15000 - 1:60000
IHC	Assay dependent

Not tested in other applications.

Calculated MW	165 kDa. ( Note )

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PROPERTIES

Form	Liquid
Buffer	0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 and 0.01% (w/v) Sodium Azide
Storage	Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration	1.02 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species	Mouse
Immunogen	Synthetic peptide: LRGAPSASRGSYTQQPR-C conjugated to KLH, corresponding to amino acids 1193-1209 of Mouse Gli2
Purification	Purified by antigen-affinity chromatography.
Conjugation	Unconjugated
Note	For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

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TARGET

Synonyms	GLI-Kruppel family member GLI2
Cellular Localization	Nucleus,Cytoplasm
Background	This protein belongs to the C2H2-type zinc finger protein subclass of the Gli family. Members of this subclass are characterized as transcription factors that bind DNA through zinc finger motifs. These motifs contain conserved H-C links. Gli family zinc finger proteins are mediators of Sonic hedgehog (Shh) signaling and they are implicated as potent oncogenes in the embryonal carcinoma cell. The protein encoded by this gene localizes to the cytoplasm and activates patched Drosophila homolog (PTCH) gene expression. Gli2 is also thought to play a role during embryogenesis. The encoded protein is associated with several phenotypes- Greig cephalopolysyndactyly syndrome, Pallister-Hall syndrome, preaxial polydactyly type IV, postaxial polydactyly types A1 and B.
Database	・ Gene ID: 14633 Gli2 ・ UniProt: Q0VGT2 Gli2

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REFERENCEE(抜粋)

Application Reference	Application/Reactivity
Morgan H et al. Methods Mol Biol 2018; (Epub) Identification of Human Cutaneous Basal Cell Carcinoma Cancer Stem Cells.	Application : IHC-Fr Reactivity : Human
Ma X et al. Neuroreport 2017; 28 (3) : 141-148 Crosstalk between Notch and Sonic hedgehog signaling in a mouse model of amyotrophic lateral sclerosis.
Colmont CS et al. Proc Natl Acad Sci U S A 2013; 110 (4) : 1434-9 CD200-expressing human basal cell carcinoma cells initiate tumor growth.	Application : IHC Reactivity : Human

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