抗GFP抗体 | Anti-GFP antibody

• 特長
• 価格
• DATA IMAGES
• 製品情報
• APPLICATION
• PROPERTIES
• TARGET
• REFERENCEE(抜粋)

特長

• 高品質の抗体です。
• 幅広い研究分野に関連する抗体を取り揃えています。
• 使用されたアプリケーションや動物種などの情報が充実しています。
• 多数の論文で使用実績がある信頼性の高い抗体です。

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価格

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DATA IMAGES

	Figure 1. Western blot of GFP recombinant protein detected with GeneTex polyclonal anti-GFP antibody. Lane 1 shows detection of a 33 kDa band corresponding to a GFP containing recombinant protein (arrowhead) expressed in HeLa cells. Lane 2 shows no staining of a mock transfected HeLa cell lysate. After blocking the membrane was probed with the primary antibody diluted to 1 μg/ml for 1 h at room temperature followed by washes and reaction with a 1:2,500 dilution of donkey anti-Goat IgG [H&L].
	Sf-1+ neurons and their processes of the ventromedial nucleus of the hypothalamus in Mus musculus (coronal view, 20X magnification). GeneTex polyclonal goat anti-GFP was used at 1:500 dilution in free floating imunnohistochemistry to detect eGFP. Fluorchrome conjugated anti-goat IgG secondary antibody was used for detection at 1:500. Sections were counterstained with DAPI. Personal Communication, Daniel A. Lee, Johns Hopkins School of Medicine, Baltimore, MD.
	Tissue: E5.5 Hex-GFP transgenic mouse embryo. Primary antibody: Goat anti-GFP was used at 1:500 dilution. Secondary antibody: Fluorchrome conjugated Anti-goat IgG secondary antibody at 1:10,000 for 45 min at RT. Staining: GFP as green fluorescent signal with DAPI blue counterstain.
	Figure 2. Immunofluorescence microscopy. GeneTex polyclonal anti-GFP antibody at a 1:1,000 dilution detects tau-GFP in cell bodies (large arrowhead) and axons of motorneurons (arrow) and interneurons (small arrowhead) in Drosophila melanogaster late stage embryonic central nervous system. Fluorochrome conjugated anti-Goat secondary antibody was used for detection at 1:300. Panel A shows a lateral view (ventral left) and Panels B and C show ventral views of whole mount embryos at 63x magnification (plus 2x digital zoom). Personal Communication, Helmata Mistry, Washington University School of Medicine, St. Louis, MO.

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製品情報

Host	Goat
Clonality	Polyclonal
Isotype	IgG
Application	WB, ICC/IF, IHC-P, IHC-Fr, IP, ELISA, IHC

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APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Dilution
WB	Assay dependent
ICC/IF	Assay dependent
IHC-P	Assay dependent
IHC-Fr	Assay dependent
IP	Assay dependent
ELISA	Assay dependent
IHC	Assay dependent

Not tested in other applications.

Specificity/Sensitivity	Anti-GFP assayed by ELISA for direct binding of antigen recognizes wild type, recombinant and enhanced forms of GFP

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PROPERTIES

Form	Liquid
Buffer	0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, containing 0.01% sodium azide
Storage	Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration	1.03 mg/ml (Please refer to the vial label for the specific concentration.)
Immunogen	Green Fluorescent Protein (GFP) fusion protein corresponding to the full length amino acid sequence (246aa) derived from the jellyfish Aequorea victoria
Purification	IgG fraction From polyclonal serum
Conjugation	Unconjugated
Note	For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

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TARGET

Background

The jellyfish Aequorea victoria contains green fluorescent protein (GFP) that emits light in the bioluminescence reaction of the animal. GFP has been used widely as a reporter protein for gene expression in eukaryotic and prokaryotic organisms, and as a protein tag in cell culture and in multicellular organisms. As a fusion tag, GFP can be used to localize proteins, to study their movement or to research the dynamics of the subcellular compartments where these proteins are targeted. GFP technology has revealed considerable new insights into the physiological activities of living cells. GFP is a 27 kDa monomeric protein, which autocatalytically forms a fluorescent pigment. The wild type protein absorbs blue light (maximally at 395nm) and emits green light (peak emission 508nm) in the absence of additional proteins, substrates, or co-factors. GFP fluorescence is stable, species independent and is suitable for a variety of applications. GFP has been used extensively as a fluorescent tag to monitor gene expression and protein localization. Moreover, other applications for GFP include its use in assessing protein protein interactions in the yeast two hybrid system, and in measuring distances between proteins in fluorescence energy transfer (FRET) experiments.

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REFERENCEE(抜粋)

Application Reference	Application/Reactivity
Christoph Schaub et al. bioRxiv 2019; (Epub) Yorkie and JNK revert syncytial muscles into myoblasts during Org-1 dependent lineage reprogramming.
Matsushita M et al. FEBS Open Bio 2019; 9 (5) : 935-946 PTPRK suppresses progression and chemo-resistance of colon cancer cells via direct inhibition of pro-oncogenic CD133.	Application : WB
Koba S et al. J Physiol 2018; (Epub) Sympathoexcitation by hypothalamic paraventricular nucleus neurons projecting to the rostral ventrolateral medulla.	Application : ICC/IF
Yao X et al. Dev Cell 2018; 45 (4) : 526-536.e5 Tild-CRISPR Allows for Efficient and Precise Gene Knockin in Mouse and Human Cells.	Application : IHC-Fr
Salameh A et al. JCI Insight 2016; 1 (10) Prohibitin/annexin 2 interaction regulates fatty acid transport in adipose tissue.	Application : ICC/IF

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