抗TNF alpha抗体 | Anti-TNF alpha antibody

• 特長
• 価格
• DATA IMAGES
• 製品情報
• APPLICATION
• PROPERTIES
• TARGET

特長

• 高品質の抗体です。
• 幅広い研究分野に関連する抗体を取り揃えています。
• 使用されたアプリケーションや動物種などの情報が充実しています。
• 多数の論文で使用実績がある信頼性の高い抗体です。

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価格

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DATA IMAGES

	GTX26671 was used at a 1:1000 dilution incubated o/n 4ºC to detect recombinant human TNF-Alpha protein by Western blot.
	Fluorescent IHC-P staining of human colon using anti-TNF alpha antibody (GTX26671) at a primary antibody dilution of 1:100.
	Immunohistochemistry using GTX26671 showing staining of formalin/PFA-fixed paraffin-embedded sections of human artery tissue sections. Sections were fixed in formaldehyde and subjected to heat mediated antigen retrieval in citrate buffer (pH 6.0). Slides were blocked for ten minutes with 1.5% serum. Primary antibody was diluted 1:100 and incubated with samples for 24 hours at 4ºC. HRP-conjugated goat anti-rabbit antibody was used as the secondary antibody.

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製品情報

Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Application	WB, IHC-P, ELISA
Reactivity	Human

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APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Dilution
WB	1:500 - 1:2,000
IHC-P	1:100 - 1:500
ELISA	1:1,000 - 1:5,000

Not tested in other applications.

Calculated MW	26 kDa. ( Note )
Specificity/Sensitivity	This antibody is primarily against mature TNF alpha protein and does not recognize human TNG-beta (lymphotoxin).

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PROPERTIES

Form	Liquid
Buffer	0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Storage	Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration	1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species	Human
Immunogen	TNF alpha antibody used to produce this IgG fraction antibody was prepared by repeated immunizations with recombinant human TNFa.
Purification	IgG fraction
Conjugation	Unconjugated
Note	For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

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TARGET

Synonyms	tumor necrosis factor , DIF , TNF-alpha , TNFA , TNFSF2 , TNLG1F
Cellular Localization	Cell membrane
Background	Tumor necrosis factor-alpha (TNF-a), also known as cachectin, is an inflammatory. TNF-a is produced by neutrophils, activated lymphocytes, macrophages, NK cells, LAK cells, Astrocytes, endothelial cells, smooth muscle cells and some transformed cells. TNF-a appears to be a major mediator of inflammatory responses and overproduction results in the extensive tissue damage associated with endotoxemia and cachexia. In addition, high serum levels of TNF are associated with septic shock, rejection of renal transplants, parasitic infections and various neoplastic diseases. TNF-a influences the growth and function of both normal and neoplastic cells. On normal cells, TNF-a exhibits growth enhancing activities for fibroblasts, T cells and B cells; differentiation of myeloid cells. On neoplastic cells, TNF-a exerts growth inhibitory activities that can be either cytostatic or cytotoxic.
Database	・ Gene ID: 7124 TNF ・ UniProt: P01375 TNF

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