抗GFP (HRP)抗体 | Anti-GFP (HRP) antibody

• 特長
• 価格
• DATA IMAGES
• 製品情報
• APPLICATION
• PROPERTIES
• TARGET
• REFERENCEE(抜粋)

特長

• 高品質の抗体です。
• 幅広い研究分野に関連する抗体を取り揃えています。
• 使用されたアプリケーションや動物種などの情報が充実しています。
• 多数の論文で使用実績がある信頼性の高い抗体です。

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価格

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DATA IMAGES

Western Blot of Anti-GFP Antibody Peroxidase Conjugate (GTX26663). Lane 1: 50ng of GFP. Primary antibody: none. Secondary antibody: Anti-GFP Antibody Peroxidase Conjugate secondary antibody was used at 1:1000 for 45 min at RT. Block: 1% BSA-TTBS 30 min at 20ºC. Predicted/Observed size: 28 kDa for GFP. Other band(s): none.

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製品情報

Host	Goat
Clonality	Polyclonal
Isotype	IgG
Application	WB, ELISA, IHC

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APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Dilution
WB	1:2000 - 1:5000
ELISA	1:10000 - 1:50000
IHC	1:500 - 1:2000

Not tested in other applications.

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PROPERTIES

Form	Liquid
Buffer	0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 and 0.01% (w/v) Gentamicin Sulfate and 10 mg/mL Bovine Serum Albumin (BSA)
Storage	Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration	1 mg/ml (Please refer to the vial label for the specific concentration.)
Immunogen	The immunogen is a Green Fluorescent Protein (GFP) fusion protein corresponding to the full length amino acid sequence (246aa) derived from the jellyfish Aequorea victoria .
Purification	Purified by antigen-affinity chromatography. From polyclonal serum
Conjugation	Horseradish peroxidase(HRP)
Note	For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

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TARGET

Background

Designed to detect GFP and its variants in ELISA (sandwich or capture), immunoblotting and immunoprecipitation Green fluorescence protein (GFP) is a 27 kDa protein derived from the jellyfish Aequorea victoria, which emits green light (emission peak at a wavelenth of 509 nm) when excited by blue light (excitation peak at a wavelenth of 395 nm). YFP differs from GFP due to a mutation at T203Y; antibodies raised against full-length GFP should also detect YFP and other variants.

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REFERENCEE(抜粋)

Application Reference	Application/Reactivity
Leen EN et al. PLoS Pathog 2016; 12 (1) : e1005379 A Conserved Interaction between a C-Terminal Motif in Norovirus VPg and the HEAT-1 Domain of eIF4G Is Essential for Translation Initiation.

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