抗GFP (TxRd)抗体 | Anti-GFP (TxRd) antibody

• 特長
• 価格
• DATA IMAGES
• 製品情報
• APPLICATION
• PROPERTIES
• TARGET

特長

• 高品質の抗体です。
• 幅広い研究分野に関連する抗体を取り揃えています。
• 使用されたアプリケーションや動物種などの情報が充実しています。
• 多数の論文で使用実績がある信頼性の高い抗体です。

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価格

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DATA IMAGES

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製品情報

Host	Goat
Clonality	Polyclonal
Isotype	IgG
Application	WB, ICC/IF, ELISA

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APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Dilution
WB	>1:10000
ICC/IF	1:500 - 1:2500
ELISA	>1:20000

Not tested in other applications.

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PROPERTIES

Form	Liquid
Buffer	0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 and 0.01% (w/v) Sodium Azide and 10 mg/mL Bovine Serum Albumin (BSA)
Storage	Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. Protect from light.
Concentration	1 mg/ml (Please refer to the vial label for the specific concentration.)
Immunogen	The immunogen is a Green Fluorescent Protein (GFP) fusion protein corresponding to the full length amino acid sequence (246aa) derived from the jellyfish Aequorea victoria.
Purification	Purified by antigen-affinity chromatography. From polyclonal serum
Conjugation	Texas Red (TxRd) Absorption : 596 nm ; Emission : 620 nm. Ratio : 2.9 molecules Texas Red per Goat IgG molecule.
Note	For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

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TARGET

Background

Designed to detect GFP and its variants in ELISA (sandwich or capture), immunoblotting and immunoprecipitation Fluorochrome conjugated anti-GFP was assayed by immunofluorescence microscopy on prokaryotic (E. Coli) and eukaryotic (CHO cells) expression systems and was shown to detect GFP containing inserts. Significant amplification of signal was detected using fluorochrome conjugated anti-GFP relative to the fluorescence of GFP alone.

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