抗p27 Kip1抗体 | Anti-p27 Kip1 antibody

• 特長
• 価格
• DATA IMAGES
• 製品情報
• APPLICATION
• PROPERTIES
• TARGET

特長

• 高品質の抗体です。
• 幅広い研究分野に関連する抗体を取り揃えています。
• 使用されたアプリケーションや動物種などの情報が充実しています。
• 多数の論文で使用実績がある信頼性の高い抗体です。

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価格

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DATA IMAGES

Western blot using GeneTex affinity purified anti-p27 antibody (GTX26547) shows detection of p27 protein in MCF7 whole cell lysate (lanes 1-3) (arrowhead). Separation was achieved using a 4-20% gradient gel. Blocking occurred using 5% BLOTTO. Primary antibody was diluted 1:500 in 1% BLOTTO. The membrane was washed and reacted with a 1:10,000 dilution of Dylight™ 800 conjugated Goat anti Rabbit IgG. Molecular weight estimation was made by comparison to prestained MW markers indicated at the left (lane M). Other detection systems will yield similar results.

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製品情報

Host	Rabbit
Clonality	Polyclonal
Application	WB, IP, ELISA
Reactivity	Human

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APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Dilution
WB	1:1000 - 1:4000
IP	1:100
ELISA	1:10000 - 1:40000

Not tested in other applications.

Calculated MW	22 kDa. ( Note )
Specificity/Sensitivity	No reactivity is observed with p27 from mouse.

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PROPERTIES

Form	Liquid
Buffer	0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 and 0.01% (w/v) Sodium Azide
Storage	Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration	80 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species	Human
Immunogen	This antibody was prepared from whole rabbit serum produced by repeated immunizations with a full length recombinant human p27 protein.
Purification	Antiserum This product was prepared from monospecific antiserum by delipidation and defibrination.
Conjugation	Unconjugated
Note	For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

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TARGET

Synonyms	cyclin dependent kinase inhibitor 1B , CDKN4 , KIP1 , MEN1B , MEN4 , P27KIP1
Cellular Localization	Nucleus,Cytoplasm,Endosome
Background	p27 Kip is a cyclin-dependent kinase inhibitor that shares a limited similarity with CDK inhibitor CDKN1A/p21. p27 binds to and prevents the activation of cyclin E-CDK2 or cyclin D-CDK4 complexes, and thus controls the cell cycle progression at G1. The degradation of this protein, which is triggered by its CDK dependent phosphorylation and subsequent ubiquitination by SCF complexes, is required for the cellular transition from quiescence to the proliferative state.
Database	・ Gene ID: 1027 CDKN1B ・ UniProt: P46527 CDKN1B

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