抗Collagen I [COL-1]抗体 | Anti-Collagen I [COL-1] antibody

• 特長
• 価格
• DATA IMAGES
• 製品情報
• APPLICATION
• PROPERTIES
• TARGET
• REFERENCEE(抜粋)

特長

• 高品質の抗体です。
• 幅広い研究分野に関連する抗体を取り揃えています。
• 使用されたアプリケーションや動物種などの情報が充実しています。
• 多数の論文で使用実績がある信頼性の高い抗体です。

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価格

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DATA IMAGES

	IHC-Fr analysis of pig tongue tissue using GTX26308 Collagen I antibody [COL-1] at 1:4,000.
	IHC-Fr analysis of human tongue tissue using GTX26308 Collagen I antibody [COL-1] at 1:2,000.

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製品情報

Host	Mouse
Clonality	Monoclonal
Clone Name	COL-1
Isotype	IgG1
Application	WB, ICC/IF, IHC-P, IHC-Fr, IP, Dot, ELISA, IHC
Reactivity	Human, Mouse, Rat, Rabbit, Bovine, Pig, Deer

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APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Dilution
WB	Assay dependent
ICC/IF	Assay dependent
IHC-P	Assay dependent
IHC-Fr	1:2000
IP	Assay dependent
Dot	Assay dependent
ELISA	Assay dependent
IHC	Assay dependent

Not tested in other applications.

Calculated MW

139 kDa. ( Note )

Specificity/Sensitivity

The antibody is reactive with the native (helical) form of collagen type I in ELISA and dot blot. The product is not reactive when tested on thermally denatured molecules. In immunohistochemical staining of acetone-fixed and unfixed frozen sections, a strong staining of connective tissue fibers is seen. Using the dot blot and ELISA techniques, the antibody shows no cross-reactivity with collagen types, II, III, IV, V, VI, VII, IX, X and XI. The epitope recognized by the antibody is sensitive to routine formalin fixation and paraffin embedding.

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PROPERTIES

Form	Liquid
Buffer	Ascites, 15 mM sodium azide
Storage	Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Antigen Species	Bovine
Immunogen	bovine skin collagen type I
Purification	Unpurified
Conjugation	Unconjugated
Note	For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

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TARGET

Synonyms	collagen type I alpha 1 chain
Background	Collagens are highly conserved throughout evolution and are characterised by an uninterrupted "Glycine X Y" triplet repeat that is a necessary part of the triple helical structure. Type I collagen (95 kDa) is found in bone, cornea, skin and tendon. Mutations in the encoding gene are associated with osteogenesis imperfecta, Ehlers Danlos syndrome, and idiopathic osteoporosis. Reciprocal translocations between chromosomes 17 and 22, where this gene and the gene for Platelet-derived growth factor beta are located, are associated with a particular type of skin tumor called dermatofibrosarcoma protuberans, resulting from unregulated expression of the growth factor.
Database	・ Gene ID: 282187 COL1A1 ・ UniProt: P02453 COL1A1

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REFERENCEE(抜粋)

Application Reference	Application/Reactivity
Chen C et al. Am J Sports Med 2020; : 363546519898519 Repair of a Meniscal Defect in a Rabbit Model Through Use of a Thermosensitive, Injectable, In Situ Crosslinked Hydrogel With Encapsulated Bone Mesenchymal Stromal Cells and Transforming Growth Factor 帣1.	Application : IHC-P Reactivity : Rabbit
Mori S et al. Nat Commun 2019; 10 (1) : 3802 Self-organized formation of developing appendages from murine pluripotent stem cells.	Application : IHC-Fr Reactivity : Mouse
Lin PH et al. Cancers (Basel) 2019; 11 (8) Isoliquiritigenin Suppresses E2-Induced Uterine Leiomyoma Growth through the Modulation of Cell Death Program and the Repression of ECM Accumulation.	Application : WB Reactivity : Rat
Wang G et al. J Orthop Surg Res 2019; 14 (1) : 204 BMSC affinity peptide-functionalized β-tricalcium phosphate scaffolds promoting repair of osteonecrosis of the femoral head.	Application : IHC-P Reactivity : Rat
Gu J et al. Evid Based Complement Alternat Med 2019; 2019 : 1358469 Huangqi Shengmai Yin Protects against Radiation-Induced Cardiac Fibrosis Injury by Regulating the TGF-帣1/Smads and MMPs.	Application : IHC-P Reactivity : Rat

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