抗beta Actin [AC-15]抗体 | Anti-beta Actin [AC-15] antibody

• 特長
• 価格
• DATA IMAGES
• 製品情報
• APPLICATION
• PROPERTIES
• TARGET
• REFERENCEE(抜粋)

特長

• 高品質の抗体です。
• 幅広い研究分野に関連する抗体を取り揃えています。
• 使用されたアプリケーションや動物種などの情報が充実しています。
• 多数の論文で使用実績がある信頼性の高い抗体です。

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価格

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DATA IMAGES

	IHC-P analysis of human heart tissue using GTX26276 beta Actin antibody [AC-15] at 1:1,000.
	WB analysis of (1) HeLa, (2) Jurkat, (3) COS7, (4) NIH3T3, (5) PC-12, (6) RAT2, (7) CHO, (8) MDBK and (9) MDCK lysates using beta Actin antibody [AC-15] at 1:5,000.
	ICC/IF analysis of FS-11 cells using GTX26276 beta Actin antibody [AC-15].
	ICC/IF analysis of HS-68 cells using GTX26276 beta Actin antibody [AC-15] at 1:1000 (green) with DAPI (blue). Cells were fixed and permeabilized with methanol followed by methanol:acetone.

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製品情報

Host	Mouse
Clonality	Monoclonal
Clone Name	AC-15
Isotype	IgG1
Application	WB, ICC/IF, IHC-P, ELISA
Reactivity	Human, Mouse, Rat, Rabbit, Sheep, Bovine, Cat, Dog, Chicken, Guinea pig, Pig, Monkey, Carp, Hirudo medicinalis

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APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Dilution
WB	1:5,000-1:10,000
ICC/IF	1:1,000-1:2,000
IHC-P	Assay dependent
ELISA	Assay dependent

Not tested in other applications.

Calculated MW	42 kDa. ( Note )
Positive Control	human or chicken fibroblast

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PROPERTIES

Form	Liquid
Buffer	Ascites, 15 mM sodium azide
Storage	Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Antigen Species	Xenopus laevis
Immunogen	slightly modified β-cytoplasmic actin N-terminal peptide, Ac-Asp-Asp-Asp-Ile-Ala-Ala-Leu-Val-Ile-Asp-Asn-Gly-Ser-Gly-Lys, conjugated to KLH.
Purification	Unpurified
Conjugation	Unconjugated
Note	For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

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TARGET

Synonyms	actin beta , BRWS1 , PS1TP5BP1
Cellular Localization	Cytoplasm, cytoskeleton,Nucleus
Background	Beta actin is one of six different actin isoforms which have been identified. Actins are highly conserved proteins that are involved in cell motility, structure and integrity.
Database	・ Gene ID: 60 ACTB ・ UniProt: P60709 ACTB

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REFERENCEE(抜粋)

Application Reference	Application/Reactivity
Saha S et al. Front Oncol 2019; 9 : 692 Transcriptomic Analysis Identifies RNA Binding Proteins as Putative Regulators of Myelopoiesis and Leukemia.	Application : WB Reactivity : Human
Wang CY et al. Int J Mol Sci 2019; 20 (20) Fetuin-A Inhibits Placental Cell Growth and Ciliogenesis in Gestational Diabetes Mellitus.	Application : WB Reactivity : Human
Buchert J et al. Int J Mol Sci 2019; 20 (17) The Role of Extracellular Matrix Expression, ERK1/2 Signaling and Cell Cohesiveness for Cartilage Yield from iPSCs.	Application : WB Reactivity : Human
Claes Z et al. J Biol Chem 2019; The antibiotic robenidine exhibits guanabenz-like cytoprotective properties by a mechanism independent of protein phosphatase PP1:PPP1R51A.	Application : WB Reactivity : Human
Diederichs S et al. Cell Mol Life Sci 2019; (Epub) Regulation of WNT5A and WNT11 during MSC in vitro chondrogenesis: WNT inhibition lowers BMP and hedgehog activity, and reduces hypertrophy.	Application : WB Reactivity : Mouse

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