抗Nestin [2Q178]抗体 | Anti-Nestin [2Q178] antibody

• 特長
• 価格
• DATA IMAGES
• 製品情報
• APPLICATION
• PROPERTIES
• TARGET
• REFERENCEE(抜粋)

特長

• 高品質の抗体です。
• 幅広い研究分野に関連する抗体を取り揃えています。
• 使用されたアプリケーションや動物種などの情報が充実しています。
• 多数の論文で使用実績がある信頼性の高い抗体です。

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価格

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DATA IMAGES

	IHC-P analysis of mouse pancreas tissue using GTX26142 Nestin antibody [2Q178].Immunoreactivity is detected in the islets of Langerhans.Antigen retrieval : Citrate buffer, pH 6.0
	IHC analysis of mouse brain tissue sections (hippocampus, CA3 region) from 21 day old mice 72 hrs after dosing with trimethyltin using GTX26142 Nestin antibody [2Q178].Blue : Nissl (rough ER)Red : nestin (Primary antibody) Green : lectin IB4 (microglia) or GFAP (glial fibrillary acidic protein, astrocytes)

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製品情報

Host	Mouse
Clonality	Monoclonal
Clone Name	2Q178
Isotype	IgG1
Application	WB, ICC/IF, IHC-P, IHC-Fr, IHC
Reactivity	Mouse, Rat, Dog, Hamster, Chicken

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APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Dilution
WB	Assay dependent
ICC/IF	1:20-1:200
IHC-P	1:20-1:200
IHC-Fr	1:20-1:200
IHC	Assay dependent
Note : ICC/IF Fix cells in 4% paraformaldehyde buffered with 50mM sodium borate, pH 9.5 IHC-P Perfuse tissue with 4% paraformaldehyde, pH 7.4 for light microscopy or with either 4% paraformaldehyde, pH 10 or 4% paraformaldehyde with 0.1% glutaraldehyde, pH 7.4 for EM. IHC-Fr Perfuse tissue with 4% paraformaldehyde, pH 7.4 for light microscopy or with either 4% paraformaldehyde, pH 10 or 4% paraformaldehyde with 0.1% glutaraldehyde, pH 7.4 for EM.

Not tested in other applications.

Calculated MW	209 kDa. ( Note )
Specificity/Sensitivity	This product does not react with Human.

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PROPERTIES

Form	Liquid
Buffer	Tris-Glycine, 0.15M sodium chloride pH 7.4, 0.09% sodium azide
Storage	Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration	Batch dependent (Please refer to the vial label for the specific concentration.)
Antigen Species	Rat
Immunogen	Nestin purified from embryonic rat spinal cord.
Purification	Purified IgG
Conjugation	Unconjugated
Note	For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

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TARGET

Background	intermediate filament protein specifically expressed in neuroepithelial stem cells; may play a role in neural cell differentiation [RGD, Feb 2006]
Database	・ Gene ID: 25491 Nes ・ UniProt: P21263 Nes

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REFERENCEE(抜粋)

Application Reference	Application/Reactivity
Berenice M?rquez-Valadez et al. Front. Neurosci. 2019; (Epub) The Systemic Administration of the Histamine H1 Receptor Antagonist/Inverse Agonist.	Application : IHC-P Reactivity : Rat
Hsieh FY et al. Biomaterials 2018; (Epub) Non-viral delivery of an optogenetic tool into cells with self-healing hydrogel.	Application : ICC/IF Reactivity : Mouse
Huang CT et al. Journal of Materials Chemistry B 2017; (44) A graphene–polyurethane composite hydrogel as a potential bioink for 3D bioprinting and differentiation of neural stem cells.	Application : ICC/IF Reactivity : Mouse
Trias E et al. JCI Insight 2017; 2 (20) Evidence for mast cells contributing to neuromuscular pathology in an inherited model of ALS.	Application : IHC-P Reactivity : Rat
Han HW et al. Colloids Surf B Biointerfaces 2017; 158 : 527-538 Chitosan derived co-spheroids of neural stem cells and mesenchymal stem cells for neural regeneration.	Application : ICC/IF Reactivity : Mouse

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