抗GLI3抗体 | Anti-GLI3 antibody

• 特長
• 価格
• DATA IMAGES
• 製品情報
• APPLICATION
• PROPERTIES
• TARGET
• REFERENCEE(抜粋)

特長

• 高品質の抗体です。
• 幅広い研究分野に関連する抗体を取り揃えています。
• 使用されたアプリケーションや動物種などの情報が充実しています。
• 多数の論文で使用実績がある信頼性の高い抗体です。

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価格

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DATA IMAGES

Western Blot of Rabbit anti-Gli-3 antibody. Lane 1: 50 kDa molecular weight marker. Lane 2: 293T cells transfected with CrkL-Flag. Lane 3: 293T cells transfected with human Gli-3. Load: 35 μg per lane. Primary antibody: Gli-3 antibody at 1:400 for overnight at 4ºC. Secondary antibody: IRDye800™ rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4ºC. Predicted/Observed size: 170-190 kDa for hGli-3. Other band(s): Non specific background ~60kDa.

Immunohistochemistry of Rabbit anti-Gli-3 antibody (GTX26050). This image tissue: human glioblastoma. Specific staining was also noted in tissue from adrenal, brain, glioblastoma, colon, heart, kidney, lung, liver, skeletal muscle, ovary, pancreas, placenta, skin, spleen, stomach, testes, thymus, thyroid, tonsil and uterus. Fixation: formalin fixed paraffin embedded. Antigen retrieval: not required. Primary antibody: Gli-3 antibody at 0.625 μg/ml for 1 h at RT. Secondary antibody: Peroxidase rabbit secondary antibody at 1:10,000 for 45 min at RT. Localization: Gli-3 is nuclear and smooth muscle. Staining: Gli-3 as precipitated red signal with hematoxylin purple nuclear counterstain.

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製品情報

Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Application	WB, IHC-P, ELISA
Reactivity	Human, Zebrafish, Dog, Chicken, Monkey, Xenopus laevis, Chimpanzee, Quail

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APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Dilution
WB	1:500 - 1:2000
IHC-P	0.5 mg/ml - 5 μg/ml
ELISA	1:6000 - 1:30000
Note : WB Recommend using nuclear extracts instead of whole cell lysates.

Not tested in other applications.

Calculated MW	170 kDa. ( Note )

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PROPERTIES

Form	Liquid
Buffer	0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 and 0.01% (w/v) Sodium Azide
Storage	Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration	1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species	Human
Immunogen	Synthetic peptide: NEDESPGQTYHRERRNA-C conjugated to KLH, corresponding to amino acids 41-57 of Human GLI3.
Purification	Purified by antigen-affinity chromatography.
Conjugation	Unconjugated
Note	For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

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TARGET

Synonyms	GLI family zinc finger 3 , ACLS , GCPS , GLI3-190 , GLI3FL , PAP-A , PAPA , PAPA1 , PAPB , PHS , PPDIV
Cellular Localization	Nucleus,Cytoplasm
Background	Gli3 is belongs to the GLI C2H2-type zinc-finger protein family and contains 5 C2H2-type zinc fingers. Gli3 is very important for normal limb and brain development and is implicated in the transduction of Shh signal. Gli3 is also expressed in a wide variety of normal adult tissues, including lung, colon, spleen, placenta, testis, and myometrium. Defects in GLI3 are the cause of Greig cephalo-poly-syndactyly syndrome (GCPS); an autosomal dominant disorder affecting limb and craniofacial development.
Database	・ Gene ID: 2737 GLI3 ・ UniProt: P10071 GLI3

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REFERENCEE(抜粋)

Application Reference	Application/Reactivity
Li N et al. Oncogene 2018; (Epub) Non-canonical activation of hedgehog in prostate cancer cells mediated by the interaction of transcriptionally active androgen receptor proteins with Gli3.	Application : WB Reactivity : Human
Bloomquist, Ryan F et al. 0 2015; Developmental plasticity of stem cells in teeth and taste bud renewal.	Application : IHC-P Reactivity : Zebrafish
Hunt R et al. Hybridoma (Larchmt) 2007; 26 (4) : 231-40 Generation and characterization of mouse monoclonal antibody 5E1 against human transcription factor GLI3.
Hunt R et al. Hybridoma (Larchmt) 2007; 26 (3) : 131-9 Generation and characterization of mouse monoclonal antibody 5E1 against human transcription factor GLI3.

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