抗c-Kit (phospho Tyr936)抗体 | Anti-c-Kit (phospho Tyr936) antibody

• 特長
• 価格
• DATA IMAGES
• 製品情報
• APPLICATION
• PROPERTIES
• TARGET

特長

• 高品質の抗体です。
• 幅広い研究分野に関連する抗体を取り揃えています。
• 使用されたアプリケーションや動物種などの情報が充実しています。
• 多数の論文で使用実績がある信頼性の高い抗体です。

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価格

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DATA IMAGES

	ICC/IF analysis of A549 cells using GTX25635 c-Kit (phospho Tyr936) antibody. Panel e is a no primary antibody control.Green : Primary antibodyBlue : NucleiRed : Actin Fixation : 4% paraformaldehydePermeabilization : 0.25% Triton X-100 for 10 minutesDilution : 1:250 dilution in 1% BSA incubated for 3 hours at room temperature
	WB analysis of M07e cells left untreated (Lane 1) or treated (Lane 2-5) with SCF using GTX25635 c-Kit (phospho Tyr936) antibody.
	WB analysis of whole cell extracts (30 μg lysate) of K562 (Lane 1) treated for 10 minutes with 100 ng/ml of SCF (Lane 2), HT 29 (Lane 3) treated for 10 minutes with 100 ng/ml of SCF (Lane 4) using GTX25635 c-Kit (phospho Tyr936) antibody. Dilution : 1:250

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製品情報

Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Application	WB, ICC/IF, FACS
Reactivity	Human

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APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Dilution
WB	1:250
ICC/IF	1:250
FACS	1:20

Not tested in other applications.

Calculated MW	110 kDa. ( Note )

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PROPERTIES

Form	Liquid
Buffer	PBS pH 7.3, 50% glycerol, 1mg/ml BSA, 0.05% sodium azide
Storage	Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration	Batch dependent (Please refer to the vial label for the specific concentration.)
Antigen Species	Human
Immunogen	The antiserum was produced against a chemically synthesized phosphopeptide derived from a region of human c-Kit that contains tyrosine 936. The sequence is conserved in cow.
Purification	Purified by antigen-affinity chromatography
Conjugation	Unconjugated
Note	For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

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TARGET

Synonyms	KIT proto-oncogene, receptor tyrosine kinase , C-Kit , CD117 , MASTC , PBT , SCFR
Background	This gene encodes the human homolog of the proto-oncogene c-kit. C-kit was first identified as the cellular homolog of the feline sarcoma viral oncogene v-kit. This protein is a type 3 transmembrane receptor for MGF (mast cell growth factor, also known as stem cell factor). Mutations in this gene are associated with gastrointestinal stromal tumors, mast cell disease, acute myelogenous lukemia, and piebaldism. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
Database	・ Gene ID: 3815 KIT ・ UniProt: P10721 KIT

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