抗Integrin beta 1 / CD29 (phospho Thr788/Thr789)抗体 | Anti-Integrin beta 1 / CD29 (phospho Thr788/Thr789) antibody

• 特長
• 価格
• DATA IMAGES
• 製品情報
• APPLICATION
• PROPERTIES
• TARGET
• REFERENCEE(抜粋)

特長

• 高品質の抗体です。
• 幅広い研究分野に関連する抗体を取り揃えています。
• 使用されたアプリケーションや動物種などの情報が充実しています。
• 多数の論文で使用実績がある信頼性の高い抗体です。

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価格

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DATA IMAGES

	WB analysis of serum-starved mitotic HeLa cells generated by treatment with 100 ng/mL taxol for 16 hours using GTX25189 Integrin beta 1 / CD29 (phospho Thr788/Thr789) antibody. The data show that only the immunogen phosphopeptide blocks the signal, demonstrating the specificity of the antibody. The membrane treated with phosphatase (Lane 5) eliminates the signal further verifying that the antibody is phospho-specific.
	WB analysis of membrane enriched extracts (30 μg lysate) of HeLa (Lane 1) treated with Paclitaxel (Lane 2), HepG2 (Lane 3) treated with Paclitaxel (Lane 4), and 10 μL of conditioned media from HepG2 cell line (Lane 5) using GTX25189 Integrin beta 1 / CD29 (phospho Thr788/Thr789) antibody. Dilution : 1:250
	ICC/IF analysis of THP-1 cells treated with 100ng/mL of IFN-gamma for 15 minutes using GTX25189 Integrin beta 1 / CD29 (phospho Thr788/Thr789) antibody. Panel e is untreated cell with no signal. Panel f represents control cells with no primary antibody to assess background.Green : Primary antibodyBlue : NucleiRed : ActinFixation : 4% paraformaldehydePermeabilization : 0.1% Triton X-100 for 10 minutes

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製品情報

Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Application	WB, ICC/IF
Reactivity	Human, Mouse, Cat

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APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Dilution
WB	1:250
ICC/IF	1:250

Not tested in other applications.

Calculated MW	88 kDa. ( Note )

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PROPERTIES

Form	Liquid
Buffer	PBS pH 7.3, 1mg/ml BSA, 50% glycerol, 0.05% sodium azide
Storage	Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration	Batch dependent (Please refer to the vial label for the specific concentration.)
Antigen Species	Human
Immunogen	The antiserum was produced against a chemically synthesized phosphopeptide derived from the region of human integrin b1 receptor that contains threonines 788 and 789 (based on Swiss Protein database, accession number P05558). The sequence is conserved in human, mouse, rat and chicken.
Purification	Purified by antigen-affinity chromatography
Conjugation	Unconjugated
Note	For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

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TARGET

Synonyms	integrin subunit beta 1 , CD29 , FNRB , GPIIA , MDF2 , MSK12 , VLA-BETA , VLAB
Cellular Localization	Cell membrane,Cell junction
Background	Integrins are heterodimeric proteins made up of alpha and beta subunits. At least 18 alpha and 8 beta subunits have been described in mammals. Integrin family members are membrane receptors involved in cell adhesion and recognition in a variety of processes including embryogenesis, hemostasis, tissue repair, immune response and metastatic diffusion of tumor cells. This gene encodes a beta subunit. Multiple alternatively spliced transcript variants which encode different protein isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
Database	・ Gene ID: 3688 ITGB1 ・ UniProt: P05556 ITGB1

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REFERENCEE(抜粋)

Application Reference	Application/Reactivity
Qin N et al. Eur J Med Chem 2016; 124 : 490-499 Synthesis and anti-metastatic effects of pregn-17(20)-en-3-amine derivatives.	Application : WB Reactivity : Human

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