抗eIF2 alpha (phospho Ser52)抗体 | Anti-eIF2 alpha (phospho Ser52) antibody

• 特長
• 価格
• DATA IMAGES
• 製品情報
• APPLICATION
• PROPERTIES
• TARGET
• REFERENCEE(抜粋)

特長

• 高品質の抗体です。
• 幅広い研究分野に関連する抗体を取り揃えています。
• 使用されたアプリケーションや動物種などの情報が充実しています。
• 多数の論文で使用実績がある信頼性の高い抗体です。

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価格

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DATA IMAGES

Rabbit anti EIF2alpha [pS52] phosphospecific polyclonal antibody. Left: Image shows immunocytochemical analysis of EIF2alpha [pS52] in rat brain (mediodorsal neocortex) sections following traumatic axonal injury (TAI) (24 h). Control (prior to injury induction) shows low basal levels of EIF2alpha [pS52]. Right: Image shows double labeling of APP (red) and EIF2alpha [pS52] (green) in neurons subjected to traumatic axonal injury (TAI). Inset (above): higher magnification of neuronal soma with TAI. EIF2alpha [pS52] immunofluorescence is localized in cytoplasm of neuronal cell body.

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製品情報

Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Application	WB, ICC/IF, IHC (Free Floating)
Reactivity	Human, Mouse, Rat, Yeast, Drosophila, Cat, Chicken, Caenorhabditis elegans, Primate

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APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Dilution
WB	Assay dependent
ICC/IF	Assay dependent
IHC (Free Floating)	Assay dependent

Not tested in other applications.

Calculated MW	36 kDa. ( Note )

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PROPERTIES

Form	Liquid
Buffer	PBS pH 7.3, 50% glycerol, 1mg/ml BSA, 0.05% sodium azide
Storage	Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration	Batch dependent (Please refer to the vial label for the specific concentration.)
Antigen Species	Human
Immunogen	The antiserum was produced against a chemically synthesized phosphopeptide derived from the region of human eIF2a that contains serine 52. This region is conserved among many species including rat, pig, cow, fruit fly and yeast.
Purification	Purified by antigen-affinity chromatography
Conjugation	Unconjugated
Note	For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

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TARGET

Synonyms	eukaryotic translation initiation factor 2 subunit alpha , EIF-2 , EIF-2A , EIF-2alpha , EIF2 , EIF2A
Background	The translation initiation factor EIF2 catalyzes the first regulated step of protein synthesis initiation, promoting the binding of the initiator tRNA to 40S ribosomal subunits. Binding occurs as a ternary complex of methionyl-tRNA, EIF2, and GTP. EIF2 is composed of 3 nonidentical subunits, the 36-kD EIF2-alpha subunit (EIF2S1), the 38-kD EIF2-beta subunit (EIF2S2; MIM 603908), and the 52-kD EIF2-gamma subunit (EIF2S3; MIM 300161). The rate of formation of the ternary complex is modulated by the phosphorylation state of EIF2-alpha (Ernst et al., 1987 [PubMed 2948954]).[supplied by OMIM, Feb 2010]
Database	・ Gene ID: 1965 EIF2S1 ・ UniProt: P05198 EIF2S1

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REFERENCEE(抜粋)

Application Reference	Application/Reactivity
Jorgensen E et al. BMC Cancer 2008; 8 : 229 Cigarette smoke induces endoplasmic reticulum stress and the unfolded protein response in normal and malignant human lung cells.	Application : WB Reactivity : Human

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